Team:Calgary/Notebook/Protocols/catalase

From 2012.igem.org

(Difference between revisions)
Line 4: Line 4:
<ol>
<ol>
-
<li>Grow cultures of <i>E. coli J04500-katG+LAA</i> and <i>J04500</i> overnight at 37*C in LB with appropriate antibiotics.</li>
+
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/onculture">Grow cultures</a> of <i>E. coli J04500-katG+LAA</i> and <i>J04500</i> overnight at 37*C in LB with appropriate antibiotics.</li>
<li>The following evening, innoculate 20 uL into 3 mL LB with varying concentrations of hydrogen peroxide (0 mM, 1 mM, 5 mM, 10 mM) and grow overnight.</li>
<li>The following evening, innoculate 20 uL into 3 mL LB with varying concentrations of hydrogen peroxide (0 mM, 1 mM, 5 mM, 10 mM) and grow overnight.</li>
<li>The following morning, observe and record culture turbidity.</li>
<li>The following morning, observe and record culture turbidity.</li>

Revision as of 03:03, 4 October 2012

Hello! iGEM Calgary's wiki functions best with Javascript enabled, especially for mobile devices. We recommend that you enable Javascript on your device for the best wiki-viewing experience. Thanks!

Catalase Assay

  1. Grow cultures of E. coli J04500-katG+LAA and J04500 overnight at 37*C in LB with appropriate antibiotics.
  2. The following evening, innoculate 20 uL into 3 mL LB with varying concentrations of hydrogen peroxide (0 mM, 1 mM, 5 mM, 10 mM) and grow overnight.
  3. The following morning, observe and record culture turbidity.