"
Team:Calgary/Notebook/Protocols
From 2012.igem.org
(Difference between revisions)
| Line 2: | Line 2: | ||
TITLE=Protocols| | TITLE=Protocols| | ||
| - | + | CONTENT = <html> | |
<p>Here is a list of all the procedures we used this summer. Each contains a description and list of materials required.</p> | <p>Here is a list of all the procedures we used this summer. Each contains a description and list of materials required.</p> | ||
<ul> | <ul> | ||
Revision as of 00:42, 2 October 2012
Hello! iGEM Calgary's wiki functions best with Javascript enabled, especially for mobile devices. We recommend that you enable Javascript on your device for the best wiki-viewing experience. Thanks!
Protocols
Here is a list of all the procedures we used this summer. Each contains a description and list of materials required.
- Preparation of Algal f2 Media
- Glass Bead Algal Transformation
- Chloroplast Isolation
- Nuclear DNA Extraction from Algae
- Plasmid Isolation (from Pseudomonas spp.)
- Genomic DNA Isolation (from Pseudomonas spp.)
- Biotinylation/Immunoprecipitation of Naphthenic Acids
- Conjugation of E. coli plasmids to Pseudomonas spp.
- Electrode Plating
- Buffer Preparation
- Electrical Settings
- Testing For Oxidation
- Plasmid Isolation (from E. coli)
- RNA Extraction
- cDNA Synthesis
- Quantitative Real Time PCR
- Testing the pBAD-lacZ Construct
- Taq PCR Protocol
- PCR Purification
- Gel Extraction
- Testing the lacI-lacZ I732901 Gene
- Bacterial Transformation
- Rehydration of Registry DNA
- Construction Techniques
- Agarose Gel Electrophresis
- LB Agar Plates
- Overnight Cultures
- Preparing Chemically Competent Cells (E. coli)
- Preparing Glycerol Stocks (E. coli)
