Team:CU-Boulder/Safety

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<center><h2>Safety</h2></center>
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<h5>1. Would any of your project ideas raise safety issues in terms of researcher, environmental, and public safety?</h5>
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Researcher safety: In its current state, the construct does not pose a threat or raise safety issues for the researchers working on it.  All work with E. coli was conducted using standard laboratory protocol and organic waste was disposed of in common biohazard procedure.  DH5-alpha E. coli cells were used for all transformations (both chemically competent and electrically competent).<br>
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Public safety: No modifications were made to the E. coli that would alter its pathogenicity to humans.  DH5-alpha cells were used, which pose no risk of disease that could be spread to the public.  The idea of the eventual use as a probiotic would need to be strictly tested in a controlled manner in the future if the construct proved to be effective.  For the IGEM project, the construct was solely a proof of concept.<br>
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Environmental safety:  Similarly to public safety, if the construct was to be tested in plants, it would need to be done in a safe manner under experimental conditions.  Considering the scope of this project, the construct was not transformed into any form of plant cell, as that would require much more advanced safety procedures and a longer time frame.<br>
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<h5> 2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?</h5> No. The parts produced by our group do not raise any safety issues.
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<h5> 3. Is there a local biosafety group, committee, or review board at your institution?</h5>
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The CU-Boulder iGEM team complied with standard Institutional Biosafety Commitee requirements. This includes but is not limited to proper decontamination procedures, sharps disposed of properly, gloves worn, working with approved agents and protocols, waste properly decontaminated, disposed, packaged, labeled, no eating, drinking, contact lenses or food storage in the lab. More details about the Institutional Biosafety Committee safety standards can be found <a href="http://www.colorado.edu/ehs/research/biological.html"> here</a>
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<p style="text-align: center;">Welcome to the CU-iGEM 2012 wiki! This is the first time the University of Colorado has competed in the International Genetically Engineered Machines competition, and we are excited to show you the science coming out of Boulder, Colorado! We are a team of five undergraduates advised by our two graduate mentors, and a smattering of post-docs. This year is also the grand opening of the brand new Biofrontiers building for bioscience, and biotechnology research. We have been fortunate enough to have lab space in the new building.</p>
 
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Use this page to answer the questions on the <a href="http://2012.igem.org/Team:CU-Boulder/Safety"/"target="_blank"> safety page </a>.
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            <div align=center> <b> Bacterial Nightlight </b> </div> <br>
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            <div align=center> <img src="http://2012.igem.org/wiki/images/9/9b/Nemo.jpg"> </div>
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            <p>Certain organisms use bioluminescence for communication, mating, or intimidating predators.
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            Our project aims to harness the bioluminescence genes, known as the Lux operon, from Aliivibrio fischeri,
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            to make a genetically engineered E. Coli machine. The six genes of the 9-kilobase Lux operon, LuxCDABE and G,
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            are regulated by LuxI and LuxR and produce blue-green light through the following reaction: </p>
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                        <b> FMNH2+O2+R-CHO → FMN + R-COOH + H2O + Light </b></p>
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          <p>Instead of simply transforming an E.Coli with the Lux operon under a single promoter, we have isolated
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          each of the six genes independently and placed them under different promoters to optimize the system
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          and produce the maximum amount of light. The entire system is regulated by a light-repressed promoter
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          so the bacteria only luminesce during nighttime. In conclusion, we have created the first ever bacteria night light!
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          Possible real world applications for this are endless and include illuminating street signs, marking hiking trails, and
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          even giving light to impoverished areas of the world that do not have access to electricity.</p>
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        <div align=center> <b> No More Biofilm! </b> </div> <br>
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            <div align=center> <img src="http://2012.igem.org/wiki/images/9/92/Mr-clean.jpg" width="306" height="208"> </div>
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            <p>This year the CU iGEM team has harnessed the quorum sensing factors endogenous in the Salmonella enterica
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            serovar typhimurium LT2 strain to detect AHLs produced by other bacteria. Neither Salmonella nor E. coli have been
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            shown to produce detectable levels of AHLs, so they were model organisms for the detection of other AHL producing
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            bacteria such as ……. AHLs are small molecules synthesized by most gram negative bacteria, and are able to freely
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            diffuse throughout the membrane. When the concentration of AHL producing bacteria in a specific area increases, the
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            total concentration of AHLs diffusing into the cytoplasm also increases. Once a threshold level of AHLs are in the cytosol,
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            transcription factors like the Vibrio fisheri LuxR or Salmonella enterica SdiA activate gene synthesis for pathogenesis factors
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            to be produced. The SdiA transcription factor has been shown to be more sensitive to lower concentrations and a greater
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            diversity of AHLs than its LuxR homolog.</p>
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Use this page to answer the questions on the <a href="http://2012.igem.org/Team:CU-Boulder/Safety"> safety page </a>.
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Latest revision as of 23:42, 7 September 2012


Safety

1. Would any of your project ideas raise safety issues in terms of researcher, environmental, and public safety?

Researcher safety: In its current state, the construct does not pose a threat or raise safety issues for the researchers working on it. All work with E. coli was conducted using standard laboratory protocol and organic waste was disposed of in common biohazard procedure. DH5-alpha E. coli cells were used for all transformations (both chemically competent and electrically competent).

Public safety: No modifications were made to the E. coli that would alter its pathogenicity to humans. DH5-alpha cells were used, which pose no risk of disease that could be spread to the public. The idea of the eventual use as a probiotic would need to be strictly tested in a controlled manner in the future if the construct proved to be effective. For the IGEM project, the construct was solely a proof of concept.

Environmental safety: Similarly to public safety, if the construct was to be tested in plants, it would need to be done in a safe manner under experimental conditions. Considering the scope of this project, the construct was not transformed into any form of plant cell, as that would require much more advanced safety procedures and a longer time frame.

2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?
No. The parts produced by our group do not raise any safety issues.

3. Is there a local biosafety group, committee, or review board at your institution?
The CU-Boulder iGEM team complied with standard Institutional Biosafety Commitee requirements. This includes but is not limited to proper decontamination procedures, sharps disposed of properly, gloves worn, working with approved agents and protocols, waste properly decontaminated, disposed, packaged, labeled, no eating, drinking, contact lenses or food storage in the lab. More details about the Institutional Biosafety Committee safety standards can be found here




Use this page to answer the questions on the safety page .