Team:CINVESTAV-IPN-UNAM MX

From 2012.igem.org

(Difference between revisions)
 
(44 intermediate revisions not shown)
Line 1: Line 1:
-
{{Template:CINVESTAV-IPN-UNAM_MXHeader}}
 
-
{{Template:CINVESTAV-IPN-UNAM_MXCSS}}
 
-
 
<html xmlns="http://www.w3.org/1999/xhtml">
<html xmlns="http://www.w3.org/1999/xhtml">
<head>
<head>
-
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
+
<style type="text/css">
-
<link rel="stylesheet" href="css/css.css" type="text/css" />
+
-
<title>Main</title>
+
-
<script src="http://ajax.googleapis.com/ajax/libs/jquery/1.7.2/jquery.min.js"></script>
+
-
</head>
+
-
<body>
+
#top-section {
 +
position: relative;
 +
height: 20px;
 +
width: 975px;
 +
margin: 0 auto 0 auto;
 +
border-left: hidden;
 +
border-right: hidden;
 +
border-bottom: hidden;
 +
}
-
<!--Content Start-->
+
body {
-
<article><h1>Project Description</h1>
+
background: #FFFFFF url(https://static.igem.org/mediawiki/2012/f/f3/Fondo_bod.jpg) repeat-x;
 +
text-align: justify;
 +
font-family: Georgia, "Times New Roman", Times, serif;
 +
font-size: 12px;
 +
color: #000000;
 +
}
-
<section>
+
.firstHeading {
-
<p>
+
margin-bottom: .1em;
-
<h2>Construction of a light and oxygen sensor to control protein expression regulation</h2>
+
visibility:hidden;
-
</p><p>
+
}
-
Rhodobacter sphaeroides (Rhb. sphaeroides) is a purple non-sulphur bacterium that belongs to the alpha-proteobacteria, the most metabolically diverse group of organisms on the planet. Part of this versatility is attributed to regulatory proteins which coordinate different metabolic states such as anaerobic photosynthesis and chemi-heterotrophy.
+
-
</p><p>
+
-
Specifically, our project is concerned with two regulatory protein systems. The first one, is the two-component global activator under anaerobiosis, PrrB/PrrA. The second system is the light and oxygen mediated repressor/anti-repressor PpsR/AppA. These act in conjunction, along with a slew of other proteins in Rhb. sphaeroides, to tightly control and balance the metabolic demands of the cell. Our team will take these two regulatory systems to express them into a Rhodopseudomonas palustris chassis. The goal is to achieve a better comprehension of the R. palustris regulatory networks through the study of their properties of genetic switches in expressed in a relative isolation within another organism, considering the interference caused by other proteins could be minimal. This will allow us to study in a more precise way how the regulatory systems sense external conditions and transduce them into alternative expression levels via signaling pathways.
+
-
</p><p>
+
-
For us to achieve this goal a cassette in which GFP expression is light-dependent by the antirepression of PpsR and oxygen dependent by the activation of PrrA/B system has been designed. All this lab work is accompanied by a computational model which, based on our experimental data, will provide a way of testing our knowledge of these systems. This in turn allows us to enhance the functionality of the device as it expresses heterologous genes in R. palustris.
+
-
</p><p>
+
-
Once we have characterized the functionality of these regulatory networks we aim to take advantage of R. palustri’s metabolic versatility, and use this functional bacteria as a microbial factory that will allow the production of products of economic value from byproducts otherwise considered pollutants such as CO2 and other industrial derivatives.
+
-
<section>
+
img[src*="/wiki/skins/common/images/wiki.png"]{
 +
    width:0;
 +
    height:0px;
 +
    padding-right:975px;
 +
    background:transparent url(https://2011.igem.org/wiki/skins/common/images/wiki.png) top left no-repeat;
 +
}
 +
</style>
 +
<title>CINVESTAV-IPN-UNAM MX</title>
 +
 
 +
<meta HTTP-EQUIV="Refresh" CONTENT="3; URL=/Team/CINVESTAV-IPN-UNAM_MX/home.htm" />
 +
 
 +
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
 +
<!--Fireworks CS6 Dreamweaver CS6 target.  Created Tue Sep 25 16:03:30 GMT-0500 (Central Daylight Time (Mexico)) 2012-->
 +
<script src="Scripts/swfobject_modified.js" type="text/javascript"></script>
 +
</head>
 +
<body bgcolor="#ffffff">
 +
<p>&nbsp;</p>
 +
<p>&nbsp;</p>
<p>
<p>
-
<h2>Safety</h2>
+
  <div><center><object classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" width="550" height="400" id="FlashID" title="Index">
-
</p><p>
+
    <param name="movie" value="https://static.igem.org/mediawiki/2012/1/1a/Rodonuevo.swf" />
-
Question 2: Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?
+
    <param name="quality" value="high" />
-
</p><p>
+
    <param name="wmode" value="opaque" />
-
No, our BioBricks parts come from Rhodobacter sphaeroides, this bacteria is non-pathogenic and it can be worked in a Biosafety level 1 laboratory, based on the CDC Biosafety in Microbiological and Biomedical Laboratories (CDC, 2007). The functionality of our parts do not produce any dangerous compound, we are working in control of gene expression. Furthermore, we used BioBricks from iGEM distribution for all our assemblies, for example GFP as reporter, these parts do not represent a biological risk.
+
    <param name="swfversion" value="6.0.65.0" />
-
<h2>References</h2>
+
    <!-- This param tag prompts users with Flash Player 6.0 r65 and higher to download the latest version of Flash Player. Delete it if you don’t want users to see the prompt. -->
-
</p><p>
+
    <param name="expressinstall" value="Scripts/expressInstall.swf" />
-
Biosafety in Microbiological and Biomedical Laboratories, (BMBL) 5th Edition (HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention and National Institutes of Health; U.S. Government Printing Office: Washington DC; 2007).  
+
    <!-- Next object tag is for non-IE browsers. So hide it from IE using IECC. -->
 +
    <!--[if !IE]>-->
 +
    <object type="application/x-shockwave-flash" data="https://static.igem.org/mediawiki/2012/1/1a/Rodonuevo.swf" width="550" height="400">
 +
      <!--<![endif]-->
 +
      <param name="quality" value="high" />
 +
      <param name="wmode" value="opaque" />
 +
      <param name="swfversion" value="6.0.65.0" />
 +
      <param name="expressinstall" value="Scripts/expressInstall.swf" />
 +
      <!-- The browser displays the following alternative content for users with Flash Player 6.0 and older. -->
 +
      <div>
 +
        <h4>Content on this page requires a newer version of Adobe Flash Player.</h4>
 +
        <p><a href="http://www.adobe.com/go/getflashplayer"><img src="http://www.adobe.com/images/shared/download_buttons/get_flash_player.gif" alt="Get Adobe Flash player" /></a></p>
 +
      </div>
 +
      <!--[if !IE]>-->
 +
    </object>
 +
    <!--<![endif]-->
 +
  </object>
</p>
</p>
-
<section>
+
<script type="text/javascript">
-
</article>
+
swfobject.registerObject("FlashID");
-
 
+
</script>
-
 
+
</center></div>
-
</nowiki>  
+
</body>
</body>
</html>
</html>

Latest revision as of 18:27, 25 October 2012

CINVESTAV-IPN-UNAM MX

 

 

Content on this page requires a newer version of Adobe Flash Player.

Get Adobe Flash player