Team:CINVESTAV-IPN-UNAM MX

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* [home.htm Home<span class="flecha">â–¼</span>]
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* [# Rhodofactory<span class="flecha">â–¼</span>]
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** [Overview.htm Overview<span class="flecha">â–¼</span>]
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The metabolic versatility of purple non-sulfur photosynthetic bacteria allows them to grow in light, darkness and with or without oxygen; all it is due to their genetic regulation mechanisms. Taking advantage of this, our project aims to build two genetic control systems based on ''R. sphaeroides'' photosynthesis cluster regulation.
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The first one is a light dependent system controlled by two proteins AppA/PpsR that works like an antirepresor/repressor mechanism, and the second one is an oxygen dependent system of two-component called PrrA/PrrB. This two devices were tested on ''R. palustris'' chassis, using a cassete in which a reporter (GFP) is regulated by external conditions that activate or repress its expresion.
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Once we have characterized the functionality of these networks, our per;spective is to develop a Rhodofactory, it means to control the produccion of differents metabolites, such as biodiesel and butanol, using simple signals.
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Latest revision as of 18:27, 25 October 2012

CINVESTAV-IPN-UNAM MX

 

 

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