Team:CD-SCU-CHINA/Notebook

From 2012.igem.org

(Difference between revisions)
Line 1: Line 1:
{{SCU-IGEM}}
{{SCU-IGEM}}
-
== * Genome extraction
+
'''* Genome extraction
* Gene isolate using PCR
* Gene isolate using PCR
* Site mutation
* Site mutation
Line 9: Line 9:
* Plasmid extraction
* Plasmid extraction
* Double Digestion for chek
* Double Digestion for chek
-
* Temperament chromatography ==
+
* Temperament chromatography'''

Revision as of 02:46, 27 September 2012

Untitled Document

* Genome extraction

  • Gene isolate using PCR
  • Site mutation
  • Enzyme digest, ligation and transformation
  • In-fusion cloning and tranformation
  • Sequensing
  • Plasmid extraction
  • Double Digestion for chek
  • Temperament chromatography