Team:Buenos Aires/DataPage

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[[File:Crossfeeding-v03-small.png | 700px]]
[[File:Crossfeeding-v03-small.png | 700px]]
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In order for the cross-feeding scheme to work we need each strain to export the amino acid they produce (either Histidine or Tryptophan). To achieve this we created a devices design to secrete to the medium an His (or Trp) rich peptides.
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{| style="width: 100%"
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| align="center" | [[File:Crossfeeding-device-design_v01.jpg]]
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| align="center" | '''Basic DNA structure of the devices with their constitutive parts'''
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|}
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* '''Kozak''' consensus sequence for initiation of translation.
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* '''Signal peptide''' that targets the product of the gene for secretion.
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* '''Trojan peptide''' to increase internalization in target cell.
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* '''Payload''': this is the exported amino acid rich domain of the protein.
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The input of the devices are ''PoPS'' and the output is secreted amino acids, so the devices are ''PoPS -> exported AA'' transducers. In principle any PoPS generating part can be used.
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You can read more about the design process [[Team:Buenos_Aires/Results/Bb1 | here]]
We built 2 '''His-export devices''', and 2 '''Trp-export devices''':
We built 2 '''His-export devices''', and 2 '''Trp-export devices''':

Latest revision as of 03:54, 27 October 2012


Data Page

Crossfeeding-v03-small.png

In order for the cross-feeding scheme to work we need each strain to export the amino acid they produce (either Histidine or Tryptophan). To achieve this we created a devices design to secrete to the medium an His (or Trp) rich peptides.

Crossfeeding-device-design v01.jpg
Basic DNA structure of the devices with their constitutive parts


  • Kozak consensus sequence for initiation of translation.
  • Signal peptide that targets the product of the gene for secretion.
  • Trojan peptide to increase internalization in target cell.
  • Payload: this is the exported amino acid rich domain of the protein.

The input of the devices are PoPS and the output is secreted amino acids, so the devices are PoPS -> exported AA transducers. In principle any PoPS generating part can be used.


You can read more about the design process here

We built 2 His-export devices, and 2 Trp-export devices:


To achive this, we had to create several new basic biobricks:

  • Kozak sequence from yeast α-factor mating pheromone (MFα1) (BBa_K792001)
  • Secretion tag from yeast α-factor mating pheromone (MFα1) (BBa_K792002)
  • HIV TAT penetratin (BBa_K792003)
  • Polyarginine trojan peptide (BBa_K792004)
  • PolyHa, a Histidine rich peptide (His-Tag) (BBa_K792005)
  • TrpZipper2, a Tryptophan rich peptide water soluble and monomeric (BBa_K792006)
  • PolyHb, a stable Histidine rich peptide designed by us (BBa_K792007)
  • PolyWb, a stable Tryptophan rich peptide designed by us(BBa_K792008)