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	We built 2 '''His-export devices''', and 2 '''Trp-export devices''':		We built 2 '''His-export devices''', and 2 '''Trp-export devices''':

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Revision as of 03:41, 27 October 2012

• iGEM BsAs
  • The team
  • Official roster
  • Members
  • Where we come from

  • Safety
  • Key questions

  • Attributions
  • Attributions

  • Sponsors
  • Institutional

  Data Page
• Project
  • Synthetic ecology
  • Overview
  • Motivation
  • Design
  • Possible applications

  • Schemes
  • Crossfeeding
  • Independent Population Control
  • Cross-population control
  • Stochastic State Transitions

  • Modeling
  • Modeling synEcology
  • Advanced modeling

  Data Page
• Results and Devices
  • Strains characterization
  • Strains description
  • Fluorescence screening
  • Screening of strain proportion
  • Auxotrophy confirmation
  • Coculture in liquid medium
  • Revertant control
  • Trp basal production
  • Growth dependence

  • BBs design
  • Trp/His export devices
  • Backup devices
  • Preparing for sending

  • Testing
  • BioBricks testing
  • SynEco testing

  Data Page
• Human practices
  • Garage Lab
  • Spreading the word
  • Solving local problems

  • EMBO
  • Seeding SynBio in Latin America

  Data Page

Data Page

We built 2 His-export devices, and 2 Trp-export devices:

• His-export I (BBa_K792009)
• Trp export I (BBa_K792010)
• His-export II (BBa_K792011)
• Trp export II (BBa_K792012)

To achive this, we had to create several new basic biobricks:

• Kozak sequence from yeast α-factor mating pheromone (MFα1) (BBa_K792001)
• Secretion tag from yeast α-factor mating pheromone (MFα1) (BBa_K792002)
• HIV TAT penetratin (BBa_K792003)
• Polyarginine trojan peptide (BBa_K792004)
• PolyHa, a Histidine rich peptide (His-Tag) (BBa_K792005)
• TrpZipper2, a Tryptophan rich peptide water soluble and monomeric (BBa_K792006)
• PolyHb, a stable Histidine rich peptide designed by us (BBa_K792007)
• PolyWb, a stable Tryptophan rich peptide designed by us(BBa_K792008)