Team:BostonU/Data

From 2012.igem.org

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<h7>We are also introducing a new promoter and gene pair to iGEM that forms a copper-dependent repressible system. We chose <i>mmoR</i> and a &sigma;<sup>54</sup> promoter associated with it from a bacterium called <i>Methylosinus trichosporium</i> OB3b. <i>mmoR</i> is a &sigma;<sup>54</sup>-dependent transcriptional activator.  
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<h7>We are also introducing a new promoter and gene pair to iGEM that forms a copper-dependent repressible system. Since the library of metal sensitive promoters was relatively small on the Registry of Standard Biological Parts, we decided to investigate a metal-dependent system. We chose <i>mmoR</i> and a &sigma;<sup>54</sup> promoter associated with it from a bacterium called <i>Methylosinus trichosporium</i> OB3b. <i>mmoR</i> is a &sigma;<sup>54</sup>-dependent transcriptional activator.  
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Revision as of 19:42, 2 October 2012

BostonU iGEM Team: Welcome


Data Collected


    Sequencing Data for Converted MoClo Parts


    Characterizing BioBrick Based Devices









    Characterizing MoClo and BioBrick Based Devices



    Characterizing New BioBrick Parts
      A New Repressible System: Copper and mmoR

        We are also introducing a new promoter and gene pair to iGEM that forms a copper-dependent repressible system. Since the library of metal sensitive promoters was relatively small on the Registry of Standard Biological Parts, we decided to investigate a metal-dependent system. We chose mmoR and a σ54 promoter associated with it from a bacterium called Methylosinus trichosporium OB3b. mmoR is a σ54-dependent transcriptional activator.



        Scanlan et. al, 2009