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Team:Bordeaux/Twelfth
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Revision as of 11:24, 24 September 2012
Day 50 : 17-09-2012
Today we did the ligations IAIB , IA'IB' , ICIE , IVAIIC5B , 5AIIICIIIEIIID , 5AIVDIVEIVC , IA IIC5B , IVA5AIVB , IVEIVC with the digestions from last friday. We inactivated the ligase after 1 hour 20°C incubation and pute the tubes overnight at 4°C
Day 51 : 18-09-2012
Today we transformed by electroporation the ligations from yesterday.
Day 52 : 19-09-2012
Today we put the transformants into preculture. Nothing on IA'IB' , ICIE , IVAIIC5B , 5AIVDIVEIVC plates so we redid ligations for those assemblies.
Day 53 : 20-09-2012
Today we did a miniprep of the precultures
Day 54 : 21-09-2012
Today we tested the plasmids on agarose gel.
