Team:Bordeaux/Safety

From 2012.igem.org

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<ul class="categories">
<ul class="categories">
<li><a href="https://2012.igem.org/Team:Bordeaux/IntroductionProject">Introduction</a></li>
<li><a href="https://2012.igem.org/Team:Bordeaux/IntroductionProject">Introduction</a></li>
-
<li><a href="https://2012.igem.org/Team:Bordeaux/BioBricks">BioBricks</a></li>
+
<li><a href="https://2012.igem.org/Team:Bordeaux/Biobricks">Biobricks</a></li>
<li><a href="https://2012.igem.org/Team:Bordeaux/Safety" class="current">Safety</a></li>
<li><a href="https://2012.igem.org/Team:Bordeaux/Safety" class="current">Safety</a></li>
</ul>
</ul>
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<h2>iGEM - Bordeaux - Safety</h2>
<h2>iGEM - Bordeaux - Safety</h2>
<p>
<p>
-
The Bordeaux team is really concerned about safety of the team members and of the environment. Here is the description of all the decisions made in order to lead our project in a safe way.
+
The Bordeaux team is really concerned about safety of the team members and of the environment. Here are our answers to the four safety questions about our iGEM project. Our experiments were led in the lab of Denis Dupuy, located in the IECB (European Institute of Chemistry and Biology) among other labs and team.  
</p>
</p>
<h2>General safety</h2>
<h2>General safety</h2>
<hr>
<hr>
</br>
</br>
-
<p>
+
<b>1. Would any of your project ideas raise safety issues in terms of:</b>
-
Our experiments were led in the lab of Denis Dupuy, located in the IECB (European Institute of Chemistry and Biology) among other labs and team. The lab has obtained all the authorizations to work with genetically modified organisms as demanded by the French and European laws.
+
<li> researcher safety ?</li>
 +
<p>Our project relies on working with DH5α strains of Escherichia coli. This bacteria is not dangerous for the operator, however it is advised to work under an extractor hood, to wear gloves and wash hands afterwards. The project aims at creating a communication path between cells. To make this work, we used genes from bacteria mainly from quorum sensing. The colors will be generated by proteins that are not dangerous (GFP, mCherry), nor are the molecules signals. The only safety issues are about the experiments themselves.
 +
</br>
 +
As in any lab, some products can be dangerous for the researcher. About our project, the most dangerous products used were ethidium bromide and chloramphenicol powder. The operator had to work wearing a lab coat, gloves and glasses under an extractor hood.
 +
To prevent burns, thick gloves are available to manipulate anything very hot or very cold.
</p>
</p>
 +
 +
<li> public safety ?</li>
<p>
<p>
-
Everyone must were a lab coat when in the lab. Enough lab coats were available for this purpose.  If necessary, gloves (latex or nitril) and glasses were available to fully protect the operator.  In addition, an extractor hood is located inside the lab to use harmful powders. Here it was used to prepare the chloramphenicol solution and gel with ethidium bromide. Thick gloves are available to work with either very hot or very cold materials to protect the operator from burning.
+
The communication between cells will be seen thanks to the expression of different colors, depending on the location of the bacteria in the plate. Thus, our transgenic bacteria are not meant to be used in any applications, so it raises no safety issues in terms of public safety.
 +
To make this work, we used genes from bacteria mainly from quorum sensing. Thus, even if our bacteria should mutate or not work as expected, no harmful molecules could be released.
</p>
</p>
 +
<li> environmental safety ?</li>
<p>
<p>
-
It is forbidden to bring any food or drinks in the lab.
+
We work with strains of <i>Escherichia coli</i>. These bacteria are genetically modified and thus resistant to some antibiotics. Then we must be careful about not releasing it in to the environment even if it cannot grow easily outside the lab. The unused cultures or remains have to be inactivated with bleach. A room is dedicated to the manipulation of bacteria. The manipulations were done in this room under another extractor hood. The incubators are also located in this room so no one has to carry cultures in and out of the room.
 +
</br>
 +
Any waste is eliminated in the right way: biological waste goes in a special bin, chemical waste in another one; glass and broken glassware are collected separately. Wastes containing ethidium bromide is collected and treated by a private society.
</p>
</p>
 +
 +
<b>2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues ? </b>
<p>
<p>
-
A room is dedicated to the manipulation of bacteria. The manipulations were done in this room under another extractor hood. The incubators are also located in this room so no one has to carry cultures in and out of the room.
+
No, all our biobricks are safe to use, and cannot produce any dangerous molecules.
-
</p>
+
-
<p>
+
-
It is forbidden to work isolated and outside the presence hours (Monday to Friday: 7a.m to 8p.m). Each team member was given a card to access the lab during these hours.
+
-
</p>
+
-
<p>
+
-
All team members have been given a copy of the safety rules of the IECB. The emergency exits are always opened and were shown to any new member of the team. In case of alarm, everyone has to go out by the closest exit. People are in charge of checking that no one is left inside the building, so it is not an individual responsibility to check for anyone else. If someone is hurt, burnt or sick, the security center is in charge of taking care of the problem and give first aid. The phone number of the security center is written above the phone and on the doors. It is advisable to record this phone number in the mobile phone memory.
+
</p>
</p>
 +
 +
<b>3. Is there a local biosafety group, committee, or review board at your institution ?</b>
<p>
<p>
 +
At our institution, no biosafety group is reviewing the project. However, we have persons responsible for the safety questions we can talk to whenever needed. These people are in charge of checking all safety rules from our country are applied. They are also in charge of checking that wastes are eliminated the right way in order to protect the environment. All the materials related to safety (extractor hoods, culture rooms…) are also verified as demanded.
 +
The lab is closed to external people, and we never work with opened windows. The lab has obtained all the authorizations to work with genetically modified organisms as demanded by the French and European laws.
 +
</br>
All safety rules about working in a laboratory and how to behave in case of accident are posted on the walls and the doors.
All safety rules about working in a laboratory and how to behave in case of accident are posted on the walls and the doors.
</p>
</p>
-
<p>
+
 
-
Benches must be cleaned before and after conducting an experiment. The material used has to be cleaned. Any waste is eliminated in the right way: biological waste goes in a special bin, chemical waste in another one; glass and broken glassware are collected separately. Unused cultures are added bleach and left for an hour before elimination. For liquids containing chemicals they are either filtered or collected for special treatment. As we also care for the environment, paper and cardboard are separated from other waste and recycled.
+
 
-
</p>
+
<b>4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions ? How could parts, devices and systems be made even safer through biosafety engineering ?</b>
-
<h2>Safety related to the iGEM project</h2>
+
<p>Some more rules are applied in our own lab. Some of them are from common sense; some other could give ideas to other teams.
-
<hr>
+
It is forbidden to bring any food or drinks in the lab.
 +
</br>
 +
It is forbidden to work isolated and outside the presence hours (Monday to Friday: 7a.m to 8p.m). If someone has to work outside the presence hours, he must not be left alone in the lab and come with a permanent from the lab (teacher, engineer, or technician). It is also possible to use a phone with a direct line to someone who will check if everything is alright and can come if something goes wrong.
</br>
</br>
-
<p>
+
The emergency exits must remain opened. In case of alarm, everyone has to go out by the closest exit. People are in charge of checking that no one is left inside the building, so it is not an individual responsibility to check for anyone else. If someone is hurt, burnt or sick, the security center is in charge of taking care of the problem and give first aid. The phone number of the security center is written above the phone and on the doors. It is advisable to record this phone number in the mobile phone memory. If it is not possible to call, start the fire alarm in order to get help.
-
Our project relies on working with DH5α strains of Escherichia coli. These bacteria are genetically modified and thus resistant to some antibiotics. Then we must be careful about not releasing it in to the environment. The unused cultures or remains have to be inactivated with bleach.
+
</p>
</p>
<p>
<p>
-
The operator is advised to wear gloves and wash hands afterwards.
+
The main point of iGEM is to make undergrad students work in a lab. However, depending of the year and the formation, some students don’t know how to behave in a lab. Some guidelines are international. Maybe writing a document about “first time in a lab” could help to avoid accidents like exposure to chemicals.
-
</p>
+
 
-
<p>
+
-
The project aims at creating a communication path between cells. The communication will be seen thanks to the expression of different colors, depending on the location of the bacteria in the plate. Thus, our transgenic bacteria are not meant to be used in any applications, so it raises no safety issues in terms of public safety.
+
-
</p>
+
-
<p>
+
-
To make this work, we used genes from bacteria mainly from quorum sensing. The colors will be generated by proteins which are not dangerous (GFP, mCherry). Thus, even if our bacteria should mutate or not work as expected, no harmful molecules could be released.
+
-
</p>
+
-
<p>
+
-
<b>1. Would any of your project ideas raise safety issues in terms of:</b>
+
-
<li> researcher safety,</li>
+
-
<li> public safety, or</li>
+
-
<li> environmental safety?</li>
+
-
</br>
+
-
<b>2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes, did you document these issues in the Registry?</b>
+
-
<li> how did you manage to handle the safety issue?</li>
+
-
<li> How could other teams learn from your experience?</li>
+
-
</br>
+
-
<b>3. Is there a local biosafety group, committee, or review board at your institution?</b>
+
-
<li> If yes, what does your local biosafety group think about your project?</li>
+
-
<li> If no, which specific biosafety rules or guidelines do you have to consider in your country?</li>
+
-
</br>
+
-
<b>4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?</b>
+
</p>
</p>
</div>
</div>

Latest revision as of 11:13, 1 October 2012

Project - iGEM Bordeaux 2012

iGEM - Bordeaux - Safety

The Bordeaux team is really concerned about safety of the team members and of the environment. Here are our answers to the four safety questions about our iGEM project. Our experiments were led in the lab of Denis Dupuy, located in the IECB (European Institute of Chemistry and Biology) among other labs and team.

General safety



1. Would any of your project ideas raise safety issues in terms of:
  • researcher safety ?
  • Our project relies on working with DH5α strains of Escherichia coli. This bacteria is not dangerous for the operator, however it is advised to work under an extractor hood, to wear gloves and wash hands afterwards. The project aims at creating a communication path between cells. To make this work, we used genes from bacteria mainly from quorum sensing. The colors will be generated by proteins that are not dangerous (GFP, mCherry), nor are the molecules signals. The only safety issues are about the experiments themselves.
    As in any lab, some products can be dangerous for the researcher. About our project, the most dangerous products used were ethidium bromide and chloramphenicol powder. The operator had to work wearing a lab coat, gloves and glasses under an extractor hood. To prevent burns, thick gloves are available to manipulate anything very hot or very cold.

  • public safety ?
  • The communication between cells will be seen thanks to the expression of different colors, depending on the location of the bacteria in the plate. Thus, our transgenic bacteria are not meant to be used in any applications, so it raises no safety issues in terms of public safety. To make this work, we used genes from bacteria mainly from quorum sensing. Thus, even if our bacteria should mutate or not work as expected, no harmful molecules could be released.

  • environmental safety ?
  • We work with strains of Escherichia coli. These bacteria are genetically modified and thus resistant to some antibiotics. Then we must be careful about not releasing it in to the environment even if it cannot grow easily outside the lab. The unused cultures or remains have to be inactivated with bleach. A room is dedicated to the manipulation of bacteria. The manipulations were done in this room under another extractor hood. The incubators are also located in this room so no one has to carry cultures in and out of the room.
    Any waste is eliminated in the right way: biological waste goes in a special bin, chemical waste in another one; glass and broken glassware are collected separately. Wastes containing ethidium bromide is collected and treated by a private society.

    2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues ?

    No, all our biobricks are safe to use, and cannot produce any dangerous molecules.

    3. Is there a local biosafety group, committee, or review board at your institution ?

    At our institution, no biosafety group is reviewing the project. However, we have persons responsible for the safety questions we can talk to whenever needed. These people are in charge of checking all safety rules from our country are applied. They are also in charge of checking that wastes are eliminated the right way in order to protect the environment. All the materials related to safety (extractor hoods, culture rooms…) are also verified as demanded. The lab is closed to external people, and we never work with opened windows. The lab has obtained all the authorizations to work with genetically modified organisms as demanded by the French and European laws.
    All safety rules about working in a laboratory and how to behave in case of accident are posted on the walls and the doors.

    4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions ? How could parts, devices and systems be made even safer through biosafety engineering ?

    Some more rules are applied in our own lab. Some of them are from common sense; some other could give ideas to other teams. It is forbidden to bring any food or drinks in the lab.
    It is forbidden to work isolated and outside the presence hours (Monday to Friday: 7a.m to 8p.m). If someone has to work outside the presence hours, he must not be left alone in the lab and come with a permanent from the lab (teacher, engineer, or technician). It is also possible to use a phone with a direct line to someone who will check if everything is alright and can come if something goes wrong.
    The emergency exits must remain opened. In case of alarm, everyone has to go out by the closest exit. People are in charge of checking that no one is left inside the building, so it is not an individual responsibility to check for anyone else. If someone is hurt, burnt or sick, the security center is in charge of taking care of the problem and give first aid. The phone number of the security center is written above the phone and on the doors. It is advisable to record this phone number in the mobile phone memory. If it is not possible to call, start the fire alarm in order to get help.

    The main point of iGEM is to make undergrad students work in a lab. However, depending of the year and the formation, some students don’t know how to behave in a lab. Some guidelines are international. Maybe writing a document about “first time in a lab” could help to avoid accidents like exposure to chemicals.