Team:Bordeaux/Ninth

From 2012.igem.org

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<li><a href="http://2012.igem.org/Team:Bordeaux/Eleventh">Eleventh Week</a></li>
<li><a href="http://2012.igem.org/Team:Bordeaux/Eleventh">Eleventh Week</a></li>
<li><a href="http://2012.igem.org/Team:Bordeaux/Twelfth">Twelfth Week</a></li>
<li><a href="http://2012.igem.org/Team:Bordeaux/Twelfth">Twelfth Week</a></li>
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<li><a href="http://2012.igem.org/Team:Bordeaux/Thirteenth">Thirteenth Week</a></li>
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Latest revision as of 18:49, 26 September 2012

Note Book - iGEM Bordeaux 2012

Day 39 : 27-08-2012


Today we put the transformants (5 coloines per plate ) on LB precuture.

Day 40 : 28-08-2012


Today we did a miniprep of the precultures.
And we runned a gel to test the ligations.
IIIAIIB , 5AIVB and IIIDIIE are OK but ICIE will have to be redone.

Day 41 : 29-08-2012


Today we did ligations for IVA5AIVB , 5AIVDIVEIVC , IIAIIBIIC(1)5B ,IAIB , 5AIIIC , ICIE.
We inactivated the ligase after 1 hour 20°C incubation and put the samples on the fridge overnight.

Day 42 : 30-08-2012


Today we purified and transformed the ligations by electoporation.

Day 43 : 31-08-2012


Today we put the transformants into a LB preculture and put them at room temperature over the weekend.