Team:Bordeaux/Fifth

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Note Book - iGEM Bordeaux 2012

Day 19 : 30-07-2012


Today we did a miniprep with the colonies put into pre-culture overnight.
We checked the plasmids concentration with the nanodrop.

Day 20 : 31-07-2012


Today we started the assembly of the first operon.

We checked the restrictions of I.A and I.C

They seem to have worked well.

Day 23 : 03-08-2012


Today we digested and ligated Biobricks IA and IB.
We then transformed the plasmids by electroporation.