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Team:Bordeaux/Eighth
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<li><a href="https://2012.igem.org/Team:Bordeaux/Seventh">Seventh Week</a></li> | <li><a href="https://2012.igem.org/Team:Bordeaux/Seventh">Seventh Week</a></li> | ||
<li><a href="https://2012.igem.org/Team:Bordeaux/Eighth" class="current">Eighth Week</a></li> | <li><a href="https://2012.igem.org/Team:Bordeaux/Eighth" class="current">Eighth Week</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Bordeaux/Ninth">Ninth Week</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Bordeaux/Tenth">Tenth Week</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Bordeaux/Eleventh">Eleventh Week</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Bordeaux/Twelfth">Twelfth Week</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Bordeaux/Thirteenth">Thirteenth Week</a></li> | ||
| + | |||
| + | |||
</ul> | </ul> | ||
Latest revision as of 18:49, 26 September 2012
Day 34 : 20-08-2012
Today we put 5 transformant colonies per plate into LB preculture And put to incubate overnight a 37°C
Day 35 : 21-08-2012
Today we did a miniprep for the tube witch had grown overnight Nanodrop results :
Day 36 : 22-08-2012
Today we tested the ligations on agarose gel IIC5B , 5AIVD , IVEIVC are OK but 5AIIIC and IIAIIB have to be remade.
Day 37 : 23-08-2012
Today we did new ligations for IAIB ICIE 5AIVB IIIAIIIB IIIEIIID. We decided to now put the ligation to incubate 1 hour at 20°C then inactivate the ligase 5 min at 80°C.
Day 38 : 24-08-2012
Today we transformed the ligations from yesterday. Before transformation we purified the DNA by ethanol precipitation. We used a new batch of electro-competent DH5α.
