Team:Bordeaux/Eighth
From 2012.igem.org
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+ | Today we tested the ligations on agarose gel | ||
+ | </br> | ||
+ | IIC5B , 5AIVD , IVEIVC are OK but 5AIIIC and IIAIIB have to be remade. | ||
- | + | </p> | |
- | + | ||
<h4>Day 37 : 23-08-2012</h4> | <h4>Day 37 : 23-08-2012</h4> | ||
<hr> | <hr> | ||
<p> | <p> | ||
+ | Today we did new ligations for IAIB ICIE 5AIVB IIIAIIIB IIIEIIID. | ||
+ | </br> | ||
+ | We decided to now put the ligation to incubate 1 hour at 20°C then inactivate the ligase 5 min at 80°C. | ||
</p> | </p> | ||
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+ | Today we transformed the ligations from yesterday. | ||
+ | </br> | ||
+ | Before transformation we purified the DNA by <a href="http://openwetware.org/wiki/Ethanol_precipitation_of_nucleic_acids">ethanol precipitation</a>. | ||
+ | </br> | ||
+ | We used a new batch of electro-competent DH5α. | ||
</p> | </p> |
Revision as of 10:52, 24 September 2012
Day 34 : 20-08-2012
Today we put 5 transformant colonies per plate into LB preculture And put to incubate overnight a 37°C
Day 35 : 21-08-2012
Today we did a miniprep for the tube witch had grown overnight Nanodrop results :
Day 36 : 22-08-2012
Today we tested the ligations on agarose gel IIC5B , 5AIVD , IVEIVC are OK but 5AIIIC and IIAIIB have to be remade.
Day 37 : 23-08-2012
Today we did new ligations for IAIB ICIE 5AIVB IIIAIIIB IIIEIIID. We decided to now put the ligation to incubate 1 hour at 20°C then inactivate the ligase 5 min at 80°C.
Day 38 : 24-08-2012
Today we transformed the ligations from yesterday. Before transformation we purified the DNA by ethanol precipitation. We used a new batch of electro-competent DH5α.