Team:Bielefeld-Germany/Test

From 2012.igem.org

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==Week 26 (10/22 - 10/28/12)==
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{{Team:Bielefeld/Head}}
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<a href="http://2012.igem.org/Team:Bielefeld-Germany/Public_relations_Overview#8"><img src="http://2012.igem-bielefeld.de/includes/wiki/images/Pfeil_links2.png"></a>
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            <span id=page-title-text>
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                How our project reaches the world
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===Monday October 22nd===
 
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* '''Team Cellulose Binding Domain:'''
 
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** Sequencing of <partinfo>BBa_K863120</partinfo> in <partinfo>BBa_J61101</partinfo> showed a deletion within the ORF of the GFP; this seemed to be the reason that the colonies are not fluorescent.
 
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** Gradient-PCR on <partinfo>BBa_I13522</partinfo> with GFP_Freiburg Pre- and Suffix Primers
 
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*** Worked fine. Clean up and digestion with ''Xba''I and ''Pst''I
 
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** Gradient-PCR on <partinfo>BBa_K863103</partinfo> with CBDcex_Freiburg and GFP_Freiburg_compl Primers
 
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*** One of 12 temperatures worked. Clean up and digestion with ''Xba''I and ''Pst''I
 
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** Gradient-PCR on <partinfo>BBa_K863113</partinfo> with CBDclos_Freiburg and GFP_Freiburg_compl Primers
 
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*** did not get product at all
 
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** Ligation of the PCR products and GFP_Freiburg and CBDcex_Freiburg-GFP_Freiburg, respectively
 
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** Transformation of both ligations
 
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** Cells plated on selection agar
 
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* '''Team Site Directed Mutagenesis:'''
 
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** Stopped Digestion of the shuttle vector, but gel went wrong and the products were lost
 
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===Tuesday October 23rd===
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<p align=justify>
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* '''Team Cellulose Binding Domain:'''
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<ul>
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** Colony PCR of B0034 + GFP_Freiburg
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*** 14/24 positive
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<li><b>We did it! About our qualification for the World Championship in Boston</b><br><br>
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*** Plated three on selection agar for plasmid isolation
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** Colony PCR of B0034 + CBDcex-GFP_Freiburg
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&#10158; <a href="http://www.laborwelt.de/aktuelles/nachrichten/2012-q4/igem-fuenf-teams-nach-boston.html">iGEM: Fünf Teams nach Boston, Laborwelt - 10/09/12</a><br>
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*** 16/24 positive
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*** Plated three on selection agar for plasmid isolation
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&#10158; <a href="http://www.biotechnologie.de/BIO/Navigation/DE/root,did=154774.html?listBlId=74462&">iGEM: Fünf Teams lösen Ticket für Boston, biotechnologie.de - 10/10/12</a></li><br>
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<li><b>Neue Züricher Zeitung</b><br><br>
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&#10158; <a href="http://www.nzz.ch/wissen/wissenschaft/arzneimittel-in-gewaessern-minimieren-1.17368069">Arzneimittel in Gewässern minimieren - 07/18/12</a></li>
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<br>
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<li><b>Neue Westfälische</b><br><br>
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&#10158; <a href="http://2012.igem.org/wiki/images/7/7e/Article_NW.png">Das Wasser wird weiblich - Article in our local newspaper Neue Westfälische, Bielefelder Tageblatt, No. 160, Author: Uwe Pollmeier - 07/12/12</a></li>
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<li><b>LIVE radio interview at Radio Hertz</b><br><br>
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&#10158; <a href="http://www.radiohertz.de/beta-site/livestream/">07/04/2012 at 6pm</a></li>
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<br>
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<li><b>WDR5 Leonardo</b><br><br>
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&#10158; <a href="http://www.wdr5.de/sendungen/leonardo/s/d/06.09.2012-16.05/b/hormone-und-betablocker-im-gewaesser.html">09/06/2012 at 4pm</a></li>
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<br>
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<li><b>Our project reaches the world </b><br><br>
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&#10158; <a href="http://www.wired.co.uk/news/archive/2012-06/25/tree-fungus-oestrogen-filter">Great Britain - Wired.co.uk - 06/25/2012</a><br>
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&#10158; <a href="http://scienceblog.com/55186/filtering-estrogen-from-drinking-water/?utm_source=feedburner&utm_medium=feed&utm_campaign=Feed%3A+scienceblogrssfeed+%28ScienceBlog.com%29">Scienceblog - 06/25/2012</a><br>
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&#10158; <a href="http://www.news-medical.net/news/20120626/Biological-filter-to-remove-estrogens-from-waste-water-and-drinking-water.aspx">Australia - News Medical - 06/26/2012</a><br>
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&#10158; <a href="http://savethewater.org/tag/bielefeld/">Los Angeles, USA - Save the Water<sup>TM</sup> - 06/26/2012</a><br>
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&#10158; <a href="http://www.eurekalert.org/pub_releases/2012-06/uob-ref062512.php">Washington, DC - EurekAlert! - 06/25/2012</a><br>
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&#10158; <a href="http://savethewater.org/tag/bielefeld/">Hollywood, USA - Save the water - 06/26/2012</a></li><br>
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&#10158; <a href="http://www.biofuelsdigest.com/biobased/2012/06/27/bielefeld-university-students-participating-in-igem-competition/">Miami, USA - BioBasedDigest - 06/27/2012</a></li><br>
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&#10158; <a href="http://phys.org/news/2012-06-estrogen.html"> Phys.org - 06/25/2012</a><br>
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&#10158; <a href="http://www.redorbit.com/news/science/1112645127/drinking-water-minus-the-estrogen/">Texas - RedOrbit - 06/25/2012</a><br>
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&#10158; <a href="http://www.provital-blog.com/en/nutrition/drinks/biological-filter-to-remove-estrogens-from-waste-water-and-drinking-water/">Provital-blog.com - 06/30/2012</a><br>
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&#10158; <a href="http://www.innovations-report.com/html/reports/awards_funding/removing_estrogen_drinking_water_197866.html"> Schmitten, Germany - innovations report - 06/25/2012</a><br>
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&#10158; <a href="http://www.pressrelations.de/new%5C/standard/result_main.cfm?r=499469&sid=&aktion=jour_pm&quelle=0&n_firmanr_=102898&pfach=1&detail=1&sektor=pm&popup_vorschau=0"> Düsseldorf, Germany - pressrelations - 06/25/2012</a><br>
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&#10158; <a href="http://www.sciencecodex.com/removing_estrogen_from_drinking_water-93912">Science Codex - 06/25/2012</a><br>
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&#10158; <a href="http://healthresearchreport.me/2012/07/01/132nd-issue-health-technology-research-synopsis-29-jun-2012/">Health Research Report - 06/29/2012</a><br>
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&#10158; <a href="http://www.scoop.it/t/syntheticbio/p/2037868685/removing-estrogen-from-drinking-water">Scoop.it! - 06/26/2012</a><br>
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&#10158; <a href="http://www.medindia.net/news/estrogen-removal-from-drinking-water-using-biological-filters-103306-1.htm?utm_source=feedburner&utm_medium=feed&utm_campaign=Feed%3A+allhealthnews+%28Medindia+Health+News%29">Chennai, India - MedINDIA Network for Health - 06/27/2012</a><br>
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&#10158; <a href="http://www.analytic-news.com/press/2012/69.html">Ober-Ramstadt, Germany - News Analytic - 06/26/2012</a><br>
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<li><b>Removing estrogen from drinking water</b><br><br>
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&#10158; <a href="http://idw-online.de/pages/de/news484982">Informationsdienst Wissenschaft - 06/25/2012</a><br>
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&#10158; <a href="http://www.bio.nrw.de/nachrichten">BIO.NRW - 06/25/2012</a><br>
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&#10158; <a href="http://www.laborpraxis.vogel.de/forschung-und-entwicklung/analytik/articles/369125/">Laborpraxis.de - 06/25/2012</a>
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&#10158; <a href="http://www.uni-protokolle.de/nachrichten/id/240229/">uni-protokolle.de - 06/25/12</a><br>
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&#10158; <a href="http://mag3.i-magazine.de/magPage/gwf_Wasser_Abwasser_Anlagen_und_Verfahren_zur_Wasseraufbereitung/Seite_28/#/28"> gwf Wasser Abwasser - 7-8/2012 p. 766-767</a></li><br>
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&#10158; <a href="http://www.bio.nrw.de/en/latest_news"> BioNRW </a></li><br>
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<li><b>Students from Bielefeld University are participating in the international iGEM competition</b><br><br>
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&#10158; <a href="http://ekvv.uni-bielefeld.de/blog/pressemitteilungen/entry/%C3%B6strogen_aus_trinkwasser_entfernen_nr">Bielefeld University - 06/25/2012 - German</a><br>
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&#10158; <a href="http://ekvv.uni-bielefeld.de/blog/uninews/entry/removing_estrogen_from_drinking_water">Bielefeld University - 06/25/2012 - English</a><br>
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&#10158; <a href="http://www.laborjournal.de/rubric/aktuell/index.lasso">Synthetische Biologie: Das Bielefelder iGEM-Team, Laborjournal - 10/2012</a></li><br>
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<li><b>Street Science</b><br><br>
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&#10158; <a href="http://www.bielefeld.de/de/pressedienst/detail.html?id=2012-08-21-12.20.37.146281"> Press release for our Street Science Day - 06/25/2012</a><br>
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&#10158; <a href="http://2012.igem.org/wiki/images/b/bf/Street_Science_NW_2708.jpg">DNA isolieren? Das kann doch jeder! (Street Science) - Article in our local newspaper Westfalen-Blatt - 08/27/12</a><br>
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&#10158; <a href="http://2012.igem.org/wiki/images/f/fe/Street_Science_NW_2708_2.jpg">Spielerische Wissenschaft (Street Science) - Article in our local newspaper Neue Westfälische, Bielefelder Tageblatt, No. 199, Author: Jonny Dähne - 08/27/12</a></li><br>
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</ul>
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</p>
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</div>
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</html>
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===Wednesday October 24th===
 
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* '''Team Cellulose Binding Domain:'''
 
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** Isolated Plasmids containing B0034 + GFP_Freiburg &
 
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*** Digestion of pooled plasmids with ''Xba''I and ''Pst''I
 
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** Isolated Plasmids containing B0034 + CBDcex-GFP_Freiburg
 
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*** Digestion of pooled plasmids with ''Xba''I and ''Pst''I
 
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** Digestion of J23100 with ''Spe''I and ''Pst''I
 
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** Clean up of inserts (B0034 + GFP_Freiburg & B0034 + CBDcex-GFP_Freiburg) and Backbone (J23100) via gel
 
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** Ligation of Backbone and the inserts
 
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** Transformation and plted on selection agar
 
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* '''Team Site Directed Mutagenesis:'''
 
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** Digestion showed that all isolated colonies still have the illegal ''Xba''I restriction side
 
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===Thursday October 25th===
 
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*'''Team Substrate Anaylsis''': The MS-MS data arrived from Marcus Persicke. To ensure our measurements and discuss what happens after the Laccase treatment and on the ESI we decided to ask an organic chemistrer so we went to Prof. Dr. Dietmar Kuck whos expert on this field. Since he had less time to discuss we have only speculativ testifies.
 
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===Friday October 26th===
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{{Team:Bielefeld/Sponsoren}}
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===Saturday October 27th===
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===Sunday October 28th===
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Revision as of 18:51, 26 October 2012

How our project reaches the world


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