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Team:Bielefeld-Germany/Results/tvel5
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Laccase TVEL5 from Trametes versicolor
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TVEL5 integrated in shuttle vector
The laccase Trametes versicolor TVEL5 BBa_K863030 was cloned with classic restriction ligation steps into the shuttle vector (BBa_K863204). This construct (BBa_K863207) was linearized and integrated into the yeast genome of P. pastoris GS115. Positive Clones with single cross over were identified by PCR as shown in the following pictures. <<<<<<<BILDER PCR (2 pieces)>>>>>>>> The clones were cultivated in minimal medium and induced with 1% (v/v) methanol twice a day for 2 days. The supernatant of the culture was examined to active laccases by testing the enzyme activity.
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