http://2012.igem.org/wiki/index.php?title=Team:Bielefeld-Germany/Results/Laccases&feed=atom&action=historyTeam:Bielefeld-Germany/Results/Laccases - Revision history2024-03-28T10:01:06ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012.igem.org/wiki/index.php?title=Team:Bielefeld-Germany/Results/Laccases&diff=298220&oldid=prevJuvoss at 03:50, 27 October 20122012-10-27T03:50:16Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>All four laccases (ECOL, BPUL, BHAL and TTHL) we accomplished to purify. Besides the successfully scale-up fermentation, those laccases could be purified in a high amount to characterize the optimal activity conditions regarding pH, temperature, buffer solutions and organic solvent resistance. Furthermore the iGEM Team Bielefeld demonstrated that the produced laccases can be immobilized maintaining their activity and the degradation capacity was screened for several micro-contaminants. These tests indicate that the ECOL and BPUL are able to degrade ethinyl estradiol and estradiol.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>All four laccases (ECOL, BPUL, BHAL and TTHL) we accomplished to purify. Besides the successfully scale-up fermentation, those laccases could be purified in a high amount to characterize the optimal activity conditions regarding pH, temperature, buffer solutions and organic solvent resistance. Furthermore the iGEM Team Bielefeld demonstrated that the produced laccases can be immobilized maintaining their activity and the degradation capacity was screened for several micro-contaminants. These tests indicate that the ECOL and BPUL are able to degrade ethinyl estradiol and estradiol.</div></td></tr>
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</table>Juvosshttp://2012.igem.org/wiki/index.php?title=Team:Bielefeld-Germany/Results/Laccases&diff=298076&oldid=prevMalakF at 03:44, 27 October 20122012-10-27T03:44:58Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[File:Bielefeld2012_Trametes_Laccase_klein.jpg|350px|center|thumb|'''Figure 1:''' The <del class="diffchange diffchange-inline">picture </del>was <del class="diffchange diffchange-inline">done </del>with [http://jmol.sourceforge.net/ Jmol-software] in combination with the data from the [http://www.rcsb.org/pdb/explore/explore.do?structureId=1GYC RCSB Protein Data Bank]]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[File:Bielefeld2012_Trametes_Laccase_klein.jpg|350px|center|thumb|'''Figure 1:''' The <ins class="diffchange diffchange-inline">figure </ins>was <ins class="diffchange diffchange-inline">designed </ins>with [http://jmol.sourceforge.net/ Jmol-software] in combination with the data from the [http://www.rcsb.org/pdb/explore/explore.do?structureId=1GYC RCSB Protein Data Bank]]]</div></td></tr>
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</table>MalakFhttp://2012.igem.org/wiki/index.php?title=Team:Bielefeld-Germany/Results/Laccases&diff=296948&oldid=prevSwiebe at 03:08, 27 October 20122012-10-27T03:08:18Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:* [https://2012.igem.org/Team:Bielefeld-Germany/Results/thermo ''Thermus thermophilus'' laccase TTHL]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:* [https://2012.igem.org/Team:Bielefeld-Germany/Results/thermo ''Thermus thermophilus'' laccase TTHL]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:* [https://2012.igem.org/Team:Bielefeld-Germany/Results/tvel5 ''Trametes versicolor'' laccase TVEL5]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:* [https://2012.igem.org/Team:Bielefeld-Germany/Results/tvel5 ''Trametes versicolor'' laccase TVEL5]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>:* [https://2012.igem.org/Team:Bielefeld-Germany/Results/comparison Comparison of the different bacterial laccases</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>:* [https://2012.igem.org/Team:Bielefeld-Germany/Results/comparison Comparison of the different bacterial laccases<ins class="diffchange diffchange-inline">]</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:* [https://2012.igem.org/Team:Bielefeld-Germany/Results/trametis Purchased positive control ''Trametes versicolor'' laccase TVEL0]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:* [https://2012.igem.org/Team:Bielefeld-Germany/Results/trametis Purchased positive control ''Trametes versicolor'' laccase TVEL0]</div></td></tr>
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</table>Swiebehttp://2012.igem.org/wiki/index.php?title=Team:Bielefeld-Germany/Results/Laccases&diff=296927&oldid=prevSwiebe at 03:07, 27 October 20122012-10-27T03:07:39Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>:* [https://2012.igem.org/Team:Bielefeld-Germany/Results/comparison Comparison of the different bacterial laccases<del class="diffchange diffchange-inline">]</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>:* [https://2012.igem.org/Team:Bielefeld-Germany/Results/comparison Comparison of the different bacterial laccases</div></td></tr>
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</table>Swiebehttp://2012.igem.org/wiki/index.php?title=Team:Bielefeld-Germany/Results/Laccases&diff=296750&oldid=prevSwiebe at 03:01, 27 October 20122012-10-27T03:01:05Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:* [https://2012.igem.org/Team:Bielefeld-Germany/Results/comparison Comparison of the different bacterial laccases]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:* [https://2012.igem.org/Team:Bielefeld-Germany/Results/comparison Comparison of the different bacterial laccases]</div></td></tr>
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</table>Swiebehttp://2012.igem.org/wiki/index.php?title=Team:Bielefeld-Germany/Results/Laccases&diff=294946&oldid=prevAgatha at 01:40, 27 October 20122012-10-27T01:40:57Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Two of these </del>(ECOL <del class="diffchange diffchange-inline">and </del>BPUL) we accomplished to purify. Besides the successfully scale-up fermentation <del class="diffchange diffchange-inline">these two </del>laccases could be purified in a high amount to characterize the optimal activity conditions regarding pH, temperature, buffer solutions and organic solvent resistance. Furthermore the iGEM Team Bielefeld demonstrated that the produced laccases can be immobilized maintaining their activity and the degradation capacity was screened for several micro-contaminants. These tests indicate that the ECOL and BPUL are able to degrade ethinyl estradiol and estradiol.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">All four laccases </ins>(ECOL<ins class="diffchange diffchange-inline">, </ins>BPUL<ins class="diffchange diffchange-inline">, BHAL and TTHL</ins>) we accomplished to purify. Besides the successfully scale-up fermentation<ins class="diffchange diffchange-inline">, those </ins>laccases could be purified in a high amount to characterize the optimal activity conditions regarding pH, temperature, buffer solutions and organic solvent resistance. Furthermore the iGEM Team Bielefeld demonstrated that the produced laccases can be immobilized maintaining their activity and the degradation capacity was screened for several micro-contaminants. These tests indicate that the ECOL and BPUL are able to degrade ethinyl estradiol and estradiol.</div></td></tr>
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</table>Agathahttp://2012.igem.org/wiki/index.php?title=Team:Bielefeld-Germany/Results/Laccases&diff=289908&oldid=prevKevinJarosch at 21:21, 26 October 20122012-10-26T21:21:33Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The iGEM Team successfully produced four active bacterial laccases and an eukaryotic laccase (click for the results):</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The iGEM Team successfully produced four active bacterial laccases and an eukaryotic laccase (click for the results):</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:* [https://2012.igem.org/Team:Bielefeld-Germany/Results/tvel5 ''Trametes versicolor'' laccase TVEL5]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:* [https://2012.igem.org/Team:Bielefeld-Germany/Results/tvel5 ''Trametes versicolor'' laccase TVEL5]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:* [https://2012.igem.org/Team:Bielefeld-Germany/Results/comparison Comparison of the different bacterial laccases]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:* [https://2012.igem.org/Team:Bielefeld-Germany/Results/comparison Comparison of the different bacterial laccases]</div></td></tr>
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</table>KevinJaroschhttp://2012.igem.org/wiki/index.php?title=Team:Bielefeld-Germany/Results/Laccases&diff=289899&oldid=prevKevinJarosch at 21:21, 26 October 20122012-10-26T21:21:01Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The iGEM Team successfully produced four active bacterial laccases and an eukaryotic laccase (click for the results):</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The iGEM Team successfully produced four active bacterial laccases and an eukaryotic laccase (click for the results):</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:* [https://2012.igem.org/Team:Bielefeld-Germany/Results/coli ''Escherichia coli'' laccase ECOL]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:* [https://2012.igem.org/Team:Bielefeld-Germany/Results/coli ''Escherichia coli'' laccase ECOL]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:* [https://2012.igem.org/Team:Bielefeld-Germany/Results/pumi''Bacillus pumilus'' laccase BPUL]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:* [https://2012.igem.org/Team:Bielefeld-Germany/Results/pumi''Bacillus pumilus'' laccase BPUL]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:* [https://2012.igem.org/Team:Bielefeld-Germany/Results/tvel5 ''Trametes versicolor'' laccase TVEL5]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:* [https://2012.igem.org/Team:Bielefeld-Germany/Results/tvel5 ''Trametes versicolor'' laccase TVEL5]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:* [https://2012.igem.org/Team:Bielefeld-Germany/Results/comparison Comparison of the different bacterial laccases]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:* [https://2012.igem.org/Team:Bielefeld-Germany/Results/comparison Comparison of the different bacterial laccases]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Two of these (ECOL and BPUL) we accomplished to purify. Besides the successfully scale-up fermentation these two laccases could be purified in a high amount to characterize the optimal activity conditions regarding pH, temperature, buffer solutions and organic solvent resistance. Furthermore the iGEM Team Bielefeld demonstrated that the produced laccases can be immobilized maintaining their activity and the degradation capacity was screened for several micro-contaminants. These tests indicate that the ECOL and BPUL are able to degrade ethinyl estradiol and estradiol.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Two of these (ECOL and BPUL) we accomplished to purify. Besides the successfully scale-up fermentation these two laccases could be purified in a high amount to characterize the optimal activity conditions regarding pH, temperature, buffer solutions and organic solvent resistance. Furthermore the iGEM Team Bielefeld demonstrated that the produced laccases can be immobilized maintaining their activity and the degradation capacity was screened for several micro-contaminants. These tests indicate that the ECOL and BPUL are able to degrade ethinyl estradiol and estradiol.</div></td></tr>
</table>KevinJaroschhttp://2012.igem.org/wiki/index.php?title=Team:Bielefeld-Germany/Results/Laccases&diff=289891&oldid=prevKevinJarosch at 21:20, 26 October 20122012-10-26T21:20:18Z<p></p>
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</table>KevinJaroschhttp://2012.igem.org/wiki/index.php?title=Team:Bielefeld-Germany/Results/Laccases&diff=289888&oldid=prevKevinJarosch at 21:20, 26 October 20122012-10-26T21:20:02Z<p></p>
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