Team:Bielefeld-Germany/Results/Laccases

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[[File:Bielefeld2012_Trametes_Laccase_klein.jpg|350px|center|thumb|'''Figure 1:''' The figure was designed with [http://jmol.sourceforge.net/ Jmol-software] in combination with the data from the [http://www.rcsb.org/pdb/explore/explore.do?structureId=1GYC RCSB Protein Data Bank]]]
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The iGEM Team successfully produced four active bacterial laccases (click for the results):
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The iGEM Team successfully produced four active bacterial laccases and an eukaryotic laccase (click for the results):
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:* [http://2012.igem.org/Team:Bielefeld-Germany/Results/coli ''Escherichia coli'' laccase ECOL]
:* [http://2012.igem.org/Team:Bielefeld-Germany/Results/coli ''Escherichia coli'' laccase ECOL]
:* [http://2012.igem.org/Team:Bielefeld-Germany/Results/pumi''Bacillus pumilus'' laccase BPUL]
:* [http://2012.igem.org/Team:Bielefeld-Germany/Results/pumi''Bacillus pumilus'' laccase BPUL]
:* [http://2012.igem.org/Team:Bielefeld-Germany/Results/halo''Bacillus halodurans'' laccase BHAL]
:* [http://2012.igem.org/Team:Bielefeld-Germany/Results/halo''Bacillus halodurans'' laccase BHAL]
:* [http://2012.igem.org/Team:Bielefeld-Germany/Results/thermo ''Thermus thermophilus'' laccase TTHL]
:* [http://2012.igem.org/Team:Bielefeld-Germany/Results/thermo ''Thermus thermophilus'' laccase TTHL]
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:* [http://2012.igem.org/Team:Bielefeld-Germany/Results/tvel5 ''Trametes versicolor'' laccase TVEL5]
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:* [http://2012.igem.org/Team:Bielefeld-Germany/Results/comparison Comparison of the different bacterial laccases]
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:* [http://2012.igem.org/Team:Bielefeld-Germany/Results/trametis Purchased positive control ''Trametes versicolor'' laccase TVEL0]
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Two of these (ECOL and BPUL) we accomplished to purify. Besides the successfully scale-up fermentation these two laccases could be purified in a high amount to characterize the optimal activity conditions regarding  pH, temperature, buffer solutions  and  organic solvent resistance. Furthermore the iGEM Team Bielefeld demonstrated that the produced laccases can be immobilized maintaining their activity and the degradation capacity was screened for several micro-contaminants. These tests indicate that the ECOL and BPUL are able to degrade ethinyl estradiol and estradiol.
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All four laccases (ECOL, BPUL, BHAL and TTHL) we accomplished to purify. Besides the successfully scale-up fermentation, those  laccases could be purified in a high amount to characterize the optimal activity conditions regarding  pH, temperature, buffer solutions  and  organic solvent resistance. Furthermore the iGEM Team Bielefeld demonstrated that the produced laccases can be immobilized maintaining their activity and the degradation capacity was screened for several micro-contaminants. These tests indicate that the ECOL and BPUL are able to degrade ethinyl estradiol and estradiol.
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The laccase of [http://2012.igem.org/Team:Bielefeld-Germany/Results/trametis ''Trametes versicolor''] is still waiting to be produced.
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Latest revision as of 03:50, 27 October 2012

Laccases


Figure 1: The figure was designed with Jmol-software in combination with the data from the RCSB Protein Data Bank


The iGEM Team successfully produced four active bacterial laccases and an eukaryotic laccase (click for the results):




All four laccases (ECOL, BPUL, BHAL and TTHL) we accomplished to purify. Besides the successfully scale-up fermentation, those laccases could be purified in a high amount to characterize the optimal activity conditions regarding pH, temperature, buffer solutions and organic solvent resistance. Furthermore the iGEM Team Bielefeld demonstrated that the produced laccases can be immobilized maintaining their activity and the degradation capacity was screened for several micro-contaminants. These tests indicate that the ECOL and BPUL are able to degrade ethinyl estradiol and estradiol.

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