Team:Bielefeld-Germany/Results/Datapage

From 2012.igem.org

(Difference between revisions)
(Created page with "{{Team:Bielefeld/Head}} <html> <div id=page-title> <span id=page-title-text> Answering important safety questions </span> ...")
(Data for our favorite new parts)
 
(14 intermediate revisions not shown)
Line 1: Line 1:
{{Team:Bielefeld/Head}}
{{Team:Bielefeld/Head}}
<html>
<html>
 +
<a href="http://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#2"><img src="http://2012.igem-bielefeld.de/includes/wiki/images/Pfeil_links2.png"></a>
         <div id=page-title>
         <div id=page-title>
             <span id=page-title-text>
             <span id=page-title-text>
-
                 Answering important safety questions
+
                 Datapage
             </span>
             </span>
         </div>
         </div>
-
</html>
 
-
<html>
 
   <div id="grey_bg">
   <div id="grey_bg">
</html>
</html>
<div style="text-align:justify;">
<div style="text-align:justify;">
-
 
+
__NOTOC__
-
<h1>Datapage</h1>
+
-
+
-
</html>
+
=== How our BioBricks work===
=== How our BioBricks work===
-
[[File:Bielefeld2012 Overview.jpg|900px|thumb|center|<div style="text-align:justify;">'''Figure 1:''' iGEM Team Bielefeld is developing a biological filter using immobilized laccases, enzymes able to radicalize and break down a broad range of aromatic substances. For the production of laccases from different bacteria, fungi and plants, two expression systems are used: ''Escherichia coli'' and the yeast ''Pichia pastoris''. Immobilization is carried out either by using CPC-silica beads or by fusing the enzymes to cellulose binding domains. The concept could be extended to other toxic pollutants in drinking and wastewater, as well as to industrial applications in paper and textile industries or even for bioremediation of contaminated soil.
+
[[File:Bielefeld2012 Overview.jpg|900px|thumb|center|'''Figure 1:''' The iGEM Team Bielefeld is developing a biological filter using immobilized laccases, enzymes able to oxidize and break down a broad range of aromatic substances. For the production of laccases from different bacteria, fungi and plants, three expression systems were used: ''Escherichia coli '' KRX and Rosetta-Gami 2 and the yeast ''Pichia pastoris''. Immobilization is carried out either by using CPC-silica beads (Controlled Pore Carrier) or by fusing the enzymes to cellulose binding domains. The concept can be extended to other toxic pollutants in drinking and wastewater, as well as to industrial applications in paper and textile industries or even for bioremediation of contaminated soil.
  ]]
  ]]
-
</div>
 
==Data for our favorite new parts==
==Data for our favorite new parts==
# <partinfo>K863000</partinfo> - '''bpul (laccase from ''Bacillus pumilus'') with T7 promoter, RBS and His-tag''': This part is used to overexpress  the laccase bpul for further purification followed by characterization of enzyme activity and substrate specificity.
# <partinfo>K863000</partinfo> - '''bpul (laccase from ''Bacillus pumilus'') with T7 promoter, RBS and His-tag''': This part is used to overexpress  the laccase bpul for further purification followed by characterization of enzyme activity and substrate specificity.
-
# <partinfo>K863005</partinfo> - '''ecol (laccase from ''E. coli'') with T7 promoter, RBS and His-tag''': This enzyme is overexpressed after induction and can be purified by His-tag. Subsequently  the laccase can be characterized regarding to enzyme activity and substrate specificity.
+
# <partinfo>K863005</partinfo> - '''ecol (laccase from ''E. coli'') with T7 promoter, RBS and His-tag''': This enzyme is overexpressed after induction with rhamnose and can be purified using its His-tag. Subsequently  the laccase can be characterized regarding to its enzyme activity and substrate specificity.
-
# <partinfo>K863204</partinfo> - '''shuttle vector pECPP11JS for site-directed recombination of genes of interest in yeast''': With this part the production and secretion of a protein of interest like laccase is possible.
+
# <partinfo>K863204</partinfo> - '''shuttle vector pECPP11JS for site-directed recombination of genes of interest in yeast''': With this part the production and secretion of a protein of interest like our laccase is possible. Protein expression is induced with methanol, selection is performed by complementation of its histidine auxotrophy.
==We have also characterized the following parts==
==We have also characterized the following parts==
# <partinfo>BBa_K863012</partinfo> - '''tthl laccase (from ''T. thermophilus'') with constitutive promoter J23100, RBS and His-tag''':  This part is used for the constitutive expression of tthl for further purification followed by characterization of enzyme activity and substrate specificity.
# <partinfo>BBa_K863012</partinfo> - '''tthl laccase (from ''T. thermophilus'') with constitutive promoter J23100, RBS and His-tag''':  This part is used for the constitutive expression of tthl for further purification followed by characterization of enzyme activity and substrate specificity.
# <partinfo>BBa_K863022</partinfo> - '''bhal laccase (''from Bacillus halodurans'') with constitutive promoter J23100, RBS and His-tag''': This part is used for the constitutive expression of bhal for further purification followed by characterization of enzyme activity and substrate specificity.
# <partinfo>BBa_K863022</partinfo> - '''bhal laccase (''from Bacillus halodurans'') with constitutive promoter J23100, RBS and His-tag''': This part is used for the constitutive expression of bhal for further purification followed by characterization of enzyme activity and substrate specificity.
-
 
+
# <partinfo>BBa_K863207</partinfo> - '''shuttle vector pECPP11JS for site-directed recombination of TVEL5 laccase in yeast:''' With this part the production and secretion of TVEL5 is possible.
-
<html>
+
-
+
-
 
+
<html>
<html>
   </div>
   </div>
 +
</div>
</html>
</html>
{{Team:Bielefeld/Sponsoren}}
{{Team:Bielefeld/Sponsoren}}

Latest revision as of 01:07, 27 October 2012

Datapage


How our BioBricks work

Figure 1: The iGEM Team Bielefeld is developing a biological filter using immobilized laccases, enzymes able to oxidize and break down a broad range of aromatic substances. For the production of laccases from different bacteria, fungi and plants, three expression systems were used: Escherichia coli KRX and Rosetta-Gami 2 and the yeast Pichia pastoris. Immobilization is carried out either by using CPC-silica beads (Controlled Pore Carrier) or by fusing the enzymes to cellulose binding domains. The concept can be extended to other toxic pollutants in drinking and wastewater, as well as to industrial applications in paper and textile industries or even for bioremediation of contaminated soil.

Data for our favorite new parts

  1. BBa_K863000 - bpul (laccase from Bacillus pumilus) with T7 promoter, RBS and His-tag: This part is used to overexpress the laccase bpul for further purification followed by characterization of enzyme activity and substrate specificity.
  2. BBa_K863005 - ecol (laccase from E. coli) with T7 promoter, RBS and His-tag: This enzyme is overexpressed after induction with rhamnose and can be purified using its His-tag. Subsequently the laccase can be characterized regarding to its enzyme activity and substrate specificity.
  3. BBa_K863204 - shuttle vector pECPP11JS for site-directed recombination of genes of interest in yeast: With this part the production and secretion of a protein of interest like our laccase is possible. Protein expression is induced with methanol, selection is performed by complementation of its histidine auxotrophy.

We have also characterized the following parts

  1. BBa_K863012 - tthl laccase (from T. thermophilus) with constitutive promoter J23100, RBS and His-tag: This part is used for the constitutive expression of tthl for further purification followed by characterization of enzyme activity and substrate specificity.
  2. BBa_K863022 - bhal laccase (from Bacillus halodurans) with constitutive promoter J23100, RBS and His-tag: This part is used for the constitutive expression of bhal for further purification followed by characterization of enzyme activity and substrate specificity.
  3. BBa_K863207 - shuttle vector pECPP11JS for site-directed recombination of TVEL5 laccase in yeast: With this part the production and secretion of TVEL5 is possible.


Promega logo 180x109.gif Logo merck.jpg BioCircle.JPG Bielefeld2012 Evonik.jpg Bielefeld2012 Baxter.png Logo knauer.jpg Logo iit.jpg Bielefeld2012 BIEKUBA.jpg Logo biometra.jpg Logo bio-nrw.png Bielefeld2012 Logo ERASynbio.jpg