Team:Bielefeld-Germany/Public relations Overview

From 2012.igem.org

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<strong>Molecular</strong>
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<strong>Student Academy</strong>
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Genetic engineering protocols
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CeBiTec pupils academy
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<strong>Production</strong>
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<strong>Poster Presentations</strong>
Upstreaming and downstreaming
Upstreaming and downstreaming
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<strong>Analytics</strong>
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<strong>Strategy Process</strong>
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Analytical methods
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“Biotechnologie 2020+” in Berlin
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<strong>Immobilization</strong>
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<strong>Press</strong>
 
 
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<strong>Material</strong>
 
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Enzymes, kits, chemicals, buffers
 
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<h3><a style="text-decoration:none; color:black;" href="https://2011.igem.org/Team:Bielefeld-Germany/Protocols/Genetics">Molecular</a></h3>
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<h3><a style="text-decoration:none; color:black;" href="https://2011.igem.org/Team:Bielefeld-Germany/Protocols/Genetics">Student Academy</a></h3>
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In this section of our protocol pages you can read more about our methods for cloning and BioBrick assembly.  
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In this section you can read more about the first CeBiTec pupils academy for synthetic biology and biotechnology witch was collaborated by the iGEM Team Bielefeld.  
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Genetic engineering is a basic tool of synthetic biology. With the help of standardized DNA building blocks (BioBricks) it is fairly easy to create new and modify existing natural systems. The methods we have used in our project to create BioBricks and to modify, mutate, transform and analyse DNA are presented in this section. Methods used: Electroporation; chemical transformation; Standard, Freiburg, Gibson and 3A BioBrick assembly; restriction analysis; colony PCR; site directed mutagenesis. <a href="https://2011.igem.org/Team:Bielefeld-Germany/Protocols/Genetics">read more</a>
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coming soon. <a href="https://2012.igem.org/Team:Bielefeld-Germany/Human_Practices/StudentAcademy">read more</a>
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<img src="https://static.igem.org/mediawiki/2012/4/4a/Bielefeld2012_Mo_Poster_300.jpg"  />
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<h3><a style="text-decoration:none; color:black;" href="https://2011.igem.org/Team:Bielefeld-Germany/Protocols/Downstream-processing">Production</a></h3>
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<h3><a style="text-decoration:none; color:black;" href="https://static.igem.org/mediawiki/2012/4/4a/Bielefeld2012_Mo_Poster_300.jpg">Poster Presentation</a></h3>
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These are the protocols for the cultivations and the downstream processing.
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On this page you can read more the presentations of our poster.
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Before one is able to work with a cell-free system based on biological material, the needed proteins have to be produced and purified first. These methods and the ones we used to characterize BioBricks in vivo are presented in this section. Used methods: Cultivations in shaking flasks and bioreactor; protein clean-up from medium, periplasm, whole cell and inclusion bodies; UF / DF; IEX; Ni-NTA columns and chromatography; recrystallization and immobilization of S-layer proteins. <a href="https://2011.igem.org/Team:Bielefeld-Germany/Protocols/Downstream-processing">read more</a>
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coming soon <a href="https://2012.igem.org/Team:Bielefeld-Germany/Human_Practices/PosterPresentations">read more</a>
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<img src="https://static.igem.org/mediawiki/2012/6/60/Bielefeld2012_Activity_300.jpg"  />
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<img src="https://static.igem.org/mediawiki/2012/1/1e/Bielefeld2012_strategy_300.jpg"  />
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<h3><a style="text-decoration:none; color:black;" href="https://2011.igem.org/Team:Bielefeld-Germany/Protocols/Analytics">Analytics</a></h3>
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<h3><a style="text-decoration:none; color:black;" href="https://static.igem.org/mediawiki/2012/1/1e/Bielefeld2012_strategy_300.jpg">Strategy Process</a></h3>
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Protocols for the analytical methods we used.  
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On 28th of June 2012 the third yearly congress of the strategy process “Biotechnologie 2020+” took place in Berlin and the iGEM Team Bielefeld was invited.  
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DNA and proteins are very small and cannot be seen by the naked eye. To control the success and the results of your upstream and downstream processes, analytical methods are necessary that give reliable results to make DNA or proteins in any way visible for you. The analytical methods we used in our project can be found in this section. Used methods: Fluorescence measurement; SDS-PAGE; MALDI-TOF; HPLC; LC-ESI-qTOF-MS/MS; molecular beacons; extraction. <a href="https://2011.igem.org/Team:Bielefeld-Germany/Protocols/Analytics">read more</a>
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coming soon  <a href="https://2012.igem.org/Team:Bielefeld-Germany/Human_Practices/StrategicProcess">read more</a>
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<h3><a style="text-decoration:none; color:black;" href="https://2011.igem.org/Team:Bielefeld-Germany/Protocols/Materials">Material</a></h3>
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<h3><a style="text-decoration:none; color:black;" href="https://2011.igem.org/Team:Bielefeld-Germany/Protocols/Materials">Press</a></h3>
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Chemicals, enzymes and kits we used in our lab work.  
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Here you can find the articles to our project.
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Chemical and biological reactions need defined conditions to work as expected. The chemicals, enzymes, kits, buffers and media we used in our project are listed in this section. <a href="https://2011.igem.org/Team:Bielefeld-Germany/Protocols/Materials">read more</a>
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coming soon <a href="https://2012.igem.org/Team:Bielefeld-Germany/Human_Practices/Press">read more</a>
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Revision as of 13:03, 12 September 2012

Student Academy

In this section you can read more about the first CeBiTec pupils academy for synthetic biology and biotechnology witch was collaborated by the iGEM Team Bielefeld.

coming soon. read more

Poster Presentation

On this page you can read more the presentations of our poster.

coming soon read more

Strategy Process

On 28th of June 2012 the third yearly congress of the strategy process “Biotechnologie 2020+” took place in Berlin and the iGEM Team Bielefeld was invited.

coming soon read more

Press

Here you can find the articles to our project.

coming soon read more


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About our Wiki

Fifteen students from different fields of study from the Bielefeld University aspired to complete a project called TOXIC COMPOUNDS IN NATURAL WATER - A CASE FOR LACCASE. In his Wiki we document our achievements and our approach.

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