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From 2012.igem.org

Activity measurements

For the measurements 96-well flat bottom microplates were used. Each well contained a total sample volume of 200 µL respectively. The sample setup was pipetted as follows:

component	concentration
buffer:	100 mM
Laccase	0,1 U
ABTS	0,1 mM
H2O	ad 200 µL

The Laccase of T. versicolor we bought for standardization was diluted in water so that 140 µL would contain 0,1 U meaning 72 x 10^-5 g of the enzyme. Please check the labjournal if Sodium-Acetate or Briton Robinson Buffer was used, respectively. The ABTS and laccase concentration optimum for standardization (so that the reaction was traceable nicely) was determinated during several experiments. Check the labjournal for further information.

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