Team:Bielefeld-Germany/Project/Achievments

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Project Achievements

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We successfully produced purified and identified an active E. coli BL21-Laccase (CueO named ECOL). Results


Bielefeld2012 haken grau.jpg
We successfully designed a shuttle vector for site-directed integration in P. pastoris which allows the production and secretion of a protein of interest. Results


Bielefeld2012 haken grau.jpg
We successfully produced purified and identified an active Bacillus pumilus DSM 27-Laccase (CotA named BPUL). Results


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We successfully produced, purified an active Thermus thermophilus HB27-Laccase (tthL named TTHL). Results


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We successfully produced an active Bacillus halodurans C-125e-Laccase (Lbh1 named BHAL). Results


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We estabilished a method for the degradation of several compounds with the help of our self-produced laccases. The LC-MS and HPLC analytics showed that our laccases are able to degrade the hormonal substrates Estradiol, Esteron and Ethinyl estradiol. Results


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We successfully immobilized Trametes versicolor-Laccase (named TVEL0) and measured their activity. Results


Bielefeld2012 haken grau.jpg
We successfully immobilized E. coli BL21-Laccase (CueO named ECOL) and measured their activity. Results


Bielefeld2012 haken grau.jpg
We successfully immobilized Bacillus pumilus DSM 27-Laccase (CotA named BPUL) and measured their activity. Results


Bielefeld2012 haken grau.jpg
We successfully improved a BioBrick by clarifying its true function. Results


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We successfully create our own database to organize our samples. Results
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We successfully the reactiopn of the degradation form ABST from the laccase and the reaction combined to the sewage plant. Results



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