Team:Bielefeld-Germany/Project/Achievments

From 2012.igem.org

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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully produced purified and identified an active ''Bacillus pumilus'' DSM 27 laccase (CotA named BPUL).<br>[https://2012.igem.org/Team:Bielefeld-Germany/Results/pumi Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully produced, purified and identified an active ''Bacillus pumilus'' DSM 27 laccase (CotA named BPUL).
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[https://2012.igem.org/Team:Bielefeld-Germany/Results/pumi Results]

Revision as of 12:43, 20 October 2012


Project Achievements

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We successfully produced purified and identified an active E. coli BL21-Laccase (CueO named ECOL).

Results


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We successfully designed a shuttle vector for site-directed integration in P. pastoris which allows the production and secretion of a protein of interest.

Results


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We successfully produced, purified and identified an active Bacillus pumilus DSM 27 laccase (CotA named BPUL).

Results


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We successfully produced, purified an active laccase from Thermus thermophilus HB27 (tthL named TTHL).

Results


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We successfully produced an active Bacillus halodurans C-125 laccase (Lbh1 named BHAL).

Results


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Successful characterization of the activity of B. pumilus laccase regarding the oxidization of ABTS under different conditions.

Results


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Successful characterization of the activity of E. coli Laccase regarding the oxidization of ABTS under different conditions.

Results


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Successful measurements of enzyme activity of T. thermophilus laccase.

Results


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Successful measurements of enzyme activity of B. halodurans laccase.

Results


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We established a method for the degradation of several compounds with the help of our self-produced laccases. The LC-MS and HPLC analysis showed that our laccases are able to degrade the hormonal substrates estradiol, estrone and ethinyl estradiol.

Results


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We successfully immobilized Trametes versicolor-laccase (named TVEL0) and measured their activity.

Results


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We successfully immobilized E. coli BL21-laccase (CueO named ECOL) and measured their activity.

Results


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We successfully immobilized Bacillus pumilus DSM 27-laccase (CotA named BPUL) and measured their activity. Results


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We successfully improved a BioBrick by clarifying its true function.

Results


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We successfully created our own database to organize our samples.

Results


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We successfully created a model which simulates the oxidation of ABTS.

Results


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We conducted a feasibility study of our project with sewage plant experts.

Results


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We did an outstanding outreach work.

Results



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