Team:Bielefeld-Germany/Project/Achievments

From 2012.igem.org

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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully improved a BioBrick by clarifying its true function. [http://partsregistry.org/Part:BBa_K392014:Experience#User_Reviews Results]
[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully improved a BioBrick by clarifying its true function. [http://partsregistry.org/Part:BBa_K392014:Experience#User_Reviews Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully create our own database to organize our samples. [http://2012.igem.org/Team:Bielefeld-Germany/Project/Database]
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Revision as of 03:30, 27 September 2012


Project Achievements

Bielefeld2012 haken grau.jpg
We successfully produced purified and identified an active E. coli BL21-Laccase (CueO named ECOL). Results


Bielefeld2012 haken grau.jpg
We successfully designed a shuttle vector for site-directed integration in P. pastoris which allows the production and secretion of a protein of interest. Results


Bielefeld2012 haken grau.jpg
We successfully produced purified and identified an active Bacillus pumilus DSM 27-Laccase (CotA named BPUL). Results


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We successfully produced, purified an active Thermus thermophilus HB27-Laccase (tthL named TTHL). Results


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We successfully produced an active Bacillus halodurans C-125e-Laccase (Lbh1 named BHAL). Results


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We estabilished a method for the degradation of several compounds with the help of our self-produced laccases. The LC-MS and HPLC analytics showed that our laccases are able to degrade the hormonal substrates Estradiol, Esteron and Ethinyl estradiol. Results


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We successfully immobilized Trametes versicolor-Laccase (named TVEL0) and measured their activity. Results


Bielefeld2012 haken grau.jpg
We successfully immobilized E. coli BL21-Laccase (CueO named ECOL) and measured their activity. Results


Bielefeld2012 haken grau.jpg
We successfully immobilized Bacillus pumilus DSM 27-Laccase (CotA named BPUL) and measured their activity. Results


Bielefeld2012 haken grau.jpg
We successfully improved a BioBrick by clarifying its true function. Results


Bielefeld2012 haken grau.jpg
We successfully create our own database to organize our samples. [1]



Promega logo 180x109.gif Logo merck.jpg BioCircle.JPG Bielefeld2012 Evonik.jpg Bielefeld2012 Baxter.png Logo knauer.jpg Logo iit.jpg Bielefeld2012 BIEKUBA.jpg Logo biometra.jpg Logo bio-nrw.png Bielefeld2012 Logo ERASynbio.jpg