Team:Bielefeld-Germany/Project/Achievments

From 2012.igem.org

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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully produced an active ''Bacillus halodurans'' C-125e-Laccase (Lbh1 named BHAL). [http://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully produced an active ''Bacillus halodurans'' C-125e-Laccase (Lbh1 named BHAL). [http://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  Successful characterization of the activity of B. pumilus Laccase regarding the oxidization of ABTS under different conditions. [http://2012.igem.org/Team:Bielefeld-Germany/Results/pumi#Activity_analysis_of_BPUL Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  Successful characterization of the activity of E. coli Laccase regarding the oxidization of ABTS under different conditions. [http://2012.igem.org/Team:Bielefeld-Germany/Results/coli#Activity_Analysis_of_ECOL Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  Successful measurements of enzyme activity of T. thermophilus Laccase. [http://2012.igem.org/Team:Bielefeld-Germany/Results/thermo#Activity_analysis_of_TTHL Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  Successful measurements of enzyme activity of B. halodurans Laccase. [http://2012.igem.org/Team:Bielefeld-Germany/Results/halo#Activity_Analysis_of_BHAL Results]
   
   
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully create our own database to organize our samples. [http://2012.igem.org/Team:Bielefeld-Germany/Project/Database Results]
[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully create our own database to organize our samples. [http://2012.igem.org/Team:Bielefeld-Germany/Project/Database Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully the reactiopn of the degradation form ABST from the laccase and the reaction combined to the sewage plant. [http://2012.igem.org/Team:Bielefeld-Germany/Project/Modell Results]
[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully the reactiopn of the degradation form ABST from the laccase and the reaction combined to the sewage plant. [http://2012.igem.org/Team:Bielefeld-Germany/Project/Modell Results]

Revision as of 03:40, 27 September 2012


Project Achievements

Bielefeld2012 haken grau.jpg
We successfully produced purified and identified an active E. coli BL21-Laccase (CueO named ECOL). Results


Bielefeld2012 haken grau.jpg
We successfully designed a shuttle vector for site-directed integration in P. pastoris which allows the production and secretion of a protein of interest. Results


Bielefeld2012 haken grau.jpg
We successfully produced purified and identified an active Bacillus pumilus DSM 27-Laccase (CotA named BPUL). Results


Bielefeld2012 haken grau.jpg
We successfully produced, purified an active Thermus thermophilus HB27-Laccase (tthL named TTHL). Results


Bielefeld2012 haken grau.jpg
We successfully produced an active Bacillus halodurans C-125e-Laccase (Lbh1 named BHAL). Results


Bielefeld2012 haken grau.jpg
Successful characterization of the activity of B. pumilus Laccase regarding the oxidization of ABTS under different conditions. Results


Bielefeld2012 haken grau.jpg
Successful characterization of the activity of E. coli Laccase regarding the oxidization of ABTS under different conditions. Results


Bielefeld2012 haken grau.jpg
Successful measurements of enzyme activity of T. thermophilus Laccase. Results


Bielefeld2012 haken grau.jpg
Successful measurements of enzyme activity of B. halodurans Laccase. Results


Bielefeld2012 haken grau.jpg
We estabilished a method for the degradation of several compounds with the help of our self-produced laccases. The LC-MS and HPLC analytics showed that our laccases are able to degrade the hormonal substrates Estradiol, Esteron and Ethinyl estradiol. Results


Bielefeld2012 haken grau.jpg
We successfully immobilized Trametes versicolor-Laccase (named TVEL0) and measured their activity. Results


Bielefeld2012 haken grau.jpg
We successfully immobilized E. coli BL21-Laccase (CueO named ECOL) and measured their activity. Results


Bielefeld2012 haken grau.jpg
We successfully immobilized Bacillus pumilus DSM 27-Laccase (CotA named BPUL) and measured their activity. Results


Bielefeld2012 haken grau.jpg
We successfully improved a BioBrick by clarifying its true function. Results


Bielefeld2012 haken grau.jpg
We successfully create our own database to organize our samples. Results


Bielefeld2012 haken grau.jpg
We successfully the reactiopn of the degradation form ABST from the laccase and the reaction combined to the sewage plant. Results



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