Team:Bielefeld-Germany/Project/Achievments

From 2012.igem.org

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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   We successfully produced purified and identified an active ''E. coli'' BL21-Laccase (CueO named ECOL). [http://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#4 Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]
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::We successfully produced, purified and identified active laccases of ''E. coli'' BL21(CueO named ECOL), ''Bacillus pumilus'' DSM 27 laccase (CotA named BPUL), ''Bacillus halodurans'' C-125 laccase (Lbh1 named BHAL) and ''Thermus thermophilus'' HB27 (tthL named TTHL).
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::[http://2012.igem.org/Team:Bielefeld-Germany/Results/coli Results ECOL]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully designed a shuttle vector for site-directed integration in ''P. pastoris'' which allows the production and secretion of a protein of interest. [http://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#11 Results]  
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::[http://2012.igem.org/Team:Bielefeld-Germany/Results/pumi Results BPUL]
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::[http://2012.igem.org/Team:Bielefeld-Germany/Results/thermo Results TTHL]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully produced purified and identified an active ''Bacillus pumilus'' DSM 27-Laccase (CotA named BPUL). [http://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#3 Results]
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::[http://2012.igem.org/Team:Bielefeld-Germany/Results/halo Results BHAL]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] 
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::We successfully designed a shuttle vector for site-directed integration in ''P. pastoris'' which allows the production and secretion of a protein of interest. We were able to show that the vector is functioning as expected. 
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully produced, purified  an active ''Thermus thermophilus'' HB27-Laccase (tthL named TTHL). [http://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#5 Results]
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::[http://2012.igem.org/Team:Bielefeld-Germany/Results/vector Results]  
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] 
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::We successfully produced the laccase TVEL5 from ''Trametes versicolor'' in ''P. pastoris'' and showed the activity of the produced enzymes.
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::[http://2012.igem.org/Team:Bielefeld-Germany/Results/tvel5 Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully produced an active ''Bacillus halodurans'' C-125e-Laccase (Lbh1 named BHAL). [http://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   
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::We successfully characterized the activity of all four bacterial laccases produced regarding the oxidization of ABTS under different conditions.
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::[http://2012.igem.org/Team:Bielefeld-Germany/Results/coli#Activity_Analysis_of_ECOL Results ECOL]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We estabilished a method for the degradation of several compounds with the help of our self-produced laccases. The LC-MS and HPLC analytics showed that our laccases are able to degrade the hormonal substrates Estradiol, Esteron and Ethinyl estradiol. [http://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
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::[http://2012.igem.org/Team:Bielefeld-Germany/Results/pumi#Activity_analysis_of_BPUL Results BPUL]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully immobilized ''Trametes versicolor''-Laccase (named TVEL0) and measured their activity. [http://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
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::[http://2012.igem.org/Team:Bielefeld-Germany/Results/thermo#Activity_analysis_of_TTHL Results TTHL]
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::[http://2012.igem.org/Team:Bielefeld-Germany/Results/halo#Activity_Analysis_of_BHAL Results BHAL]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully immobilized ''E. coli'' BL21-Laccase (CueO named ECOL) and measured their activity. [http://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]
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::We established a method for the degradation of several compounds with the help of our self-produced laccases. The HPLC analysis showed that our produced bacterial laccases are able to degrade estradiol with and without addition of ABTS. BPUL is able to degrade ethinyl estradiol with addition of ABTS. LC-MS measurements showed degradation products which could not be identified but were further analyzed using MS/MS. The resulting products have been discussed with an organic chemist.
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::[http://2012.igem.org/Team:Bielefeld-Germany/Results/substrate Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully immobilized ''Bacillus pumilus'' DSM 27-Laccase (CotA named BPUL) and measured their activity. [http://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  
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::We successfully immobilized ''Trametes versicolor'' laccase (named TVEL0), ''E. coli'' BL21 laccase (CueO named ECOL) and ''Bacillus pumilus'' DSM 27 laccase (CotA named BPUL) and measured their activity.  
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::[http://2012.igem.org/Team:Bielefeld-Germany/Results/immo Results TVEL0]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully improved a BioBrick by clarifying its true function. [http://partsregistry.org/Part:BBa_K392014:Experience#User_Reviews Results]
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::[http://2012.igem.org/Team:Bielefeld-Germany/Results/immo#Immobilization_of_purified_ECOL.2C_BPUL.2C_BHAL_and_TTHL_and_Activity_Tests Results ECOL, BPUL, BHAL and TTHL]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]
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::We successfully created a model which simulates the required laccase amount to degrade harmful substances under real sewage plant conditions.
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully create our own database to organize our samples. [http://2012.igem.org/Team:Bielefeld-Germany/Project/Database]
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::[http://2012.igem.org/Team:Bielefeld-Germany/Modell Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]
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::We conducted a feasibility study of our project with sewage plant experts.
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::[http://2012.igem.org/Team:Bielefeld-Germany/Sewage_treatment_plant Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]
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::We successfully created our own database to organize our samples.
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::[http://2012.igem.org/Team:Bielefeld-Germany/Project/Database Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]
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::We successfully improved a BioBrick by clarifying its true function.
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::[http://partsregistry.org/Part:BBa_K392014:Experience#User_Reviews Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]
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::We did an outstanding outreach work.
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::[http://2012.igem.org/Team:Bielefeld-Germany/Public_relations_Overview Results]
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<br style="clear: both" />
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[[File:Bielefeld-Germany2012_Goldmedaillie.png‎|60px|frameless|left]]
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:::We won a gold medal at the Europe Regional Jamboree.
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[[File:Bielefeld-Germany2012_Qualified_stemp.png|60px|frameless|left]]
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:::We qualified for the world campionship
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<br style="clear: both" />
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</b>

Latest revision as of 23:33, 2 December 2012


Project Achievements

Bielefeld2012 haken grau.jpg
We successfully produced, purified and identified active laccases of E. coli BL21(CueO named ECOL), Bacillus pumilus DSM 27 laccase (CotA named BPUL), Bacillus halodurans C-125 laccase (Lbh1 named BHAL) and Thermus thermophilus HB27 (tthL named TTHL).
Results ECOL
Results BPUL
Results TTHL
Results BHAL
Bielefeld2012 haken grau.jpg
We successfully designed a shuttle vector for site-directed integration in P. pastoris which allows the production and secretion of a protein of interest. We were able to show that the vector is functioning as expected.
Results
Bielefeld2012 haken grau.jpg
We successfully produced the laccase TVEL5 from Trametes versicolor in P. pastoris and showed the activity of the produced enzymes.
Results
Bielefeld2012 haken grau.jpg
We successfully characterized the activity of all four bacterial laccases produced regarding the oxidization of ABTS under different conditions.
Results ECOL
Results BPUL
Results TTHL
Results BHAL
Bielefeld2012 haken grau.jpg
We established a method for the degradation of several compounds with the help of our self-produced laccases. The HPLC analysis showed that our produced bacterial laccases are able to degrade estradiol with and without addition of ABTS. BPUL is able to degrade ethinyl estradiol with addition of ABTS. LC-MS measurements showed degradation products which could not be identified but were further analyzed using MS/MS. The resulting products have been discussed with an organic chemist.
Results
Bielefeld2012 haken grau.jpg
We successfully immobilized Trametes versicolor laccase (named TVEL0), E. coli BL21 laccase (CueO named ECOL) and Bacillus pumilus DSM 27 laccase (CotA named BPUL) and measured their activity.
Results TVEL0
Results ECOL, BPUL, BHAL and TTHL
Bielefeld2012 haken grau.jpg
We successfully created a model which simulates the required laccase amount to degrade harmful substances under real sewage plant conditions.
Results
Bielefeld2012 haken grau.jpg
We conducted a feasibility study of our project with sewage plant experts.
Results
Bielefeld2012 haken grau.jpg
We successfully created our own database to organize our samples.
Results
Bielefeld2012 haken grau.jpg
We successfully improved a BioBrick by clarifying its true function.
Results
Bielefeld2012 haken grau.jpg
We did an outstanding outreach work.
Results


Bielefeld-Germany2012 Goldmedaillie.png
We won a gold medal at the Europe Regional Jamboree.


Bielefeld-Germany2012 Qualified stemp.png
We qualified for the world campionship



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