Team:Bielefeld-Germany/Project/Achievments

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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] Successful immobilization of ''Bacillus pumilus'' DSM 27 -Laccase (CotA named BPUL). [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] Successful immobilization of ''Bacillus pumilus'' DSM 27-Laccase (CotA named BPUL). [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]

Revision as of 23:45, 26 September 2012


Project Achievments

Bielefeld2012 haken grau.jpg
We successfully produced purified and identified an active E. coli BL21-Laccase (CueO named ECOL). Results


Bielefeld2012 haken grau.jpg
We successfully designed a shuttle vector for site-directed integration in P. pastoris which allows the production and secretion of a protein of interest. Results


Bielefeld2012 haken grau.jpg
We successfully produced purified and identified an active Bacillus pumilus DSM 27-Laccase (CotA named BPUL). Results


Bielefeld2012 haken grau.jpg
We successfully produced, purified an active Thermus thermophilus HB27-Laccase (tthL named TTHL). Results


Bielefeld2012 haken grau.jpg
We successfully produced an active Bacillus halodurans C-125e-Laccase (Lbh1 named BHAL). Results


Bielefeld2012 haken grau.jpg
We estabilished a method for the degradation of several compounds with the help of our self-produced laccases. The LC-MS and HPLC analytics showed that our laccases are able to degrade the hormonal substrates Estradiol, Esteron and Ethinyl estradiol Results


Bielefeld2012 haken grau.jpg
Successful immobilization of Bacillus pumilus DSM 27-Laccase (CotA named BPUL). Results


Bielefeld2012 haken grau.jpg
Successful immobilization of E. coli BL21-Laccase (CueO named ECOL). Results


Bielefeld2012 haken grau.jpg
Successful immobilization of Trametes versicolor-Laccase (named TVEL0). Results




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