Team:Bielefeld-Germany/Project/Achievments

From 2012.igem.org

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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully produced purified and identified an active ''E. coli'' BL21- Laccase (CueO named ECOL) [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#4 Results]  
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully produced purified and identified an active ''E. coli'' BL21-Laccase (CueO named ECOL). [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#4 Results]  
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully designed a shuttle vector for site-directed integration in ''P. pastoris'' which allows the production and secretion of a protein of interest [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#11 Results]  
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully designed a shuttle vector for site-directed integration in ''P. pastoris'' which allows the production and secretion of a protein of interest. [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#11 Results]  
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully produced purified and identified an active ''Bacillus pumilus'' DSM 27 -Laccase (CotA named BPUL) [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#3 Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully produced purified and identified an active ''Bacillus pumilus'' DSM 27-Laccase (CotA named BPUL). [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#3 Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully produced, purified  an active ''Thermus thermophilus'' HB27- Laccase (tthL named TTHL) [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#5 Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully produced, purified  an active ''Thermus thermophilus'' HB27-Laccase (tthL named TTHL). [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#5 Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully produced an active ''Bacillus halodurans'' C-125e -Laccase (Lbh1 named BHAL) [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully produced an active ''Bacillus halodurans'' C-125e-Laccase (Lbh1 named BHAL). [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
   
   
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We estabilished a method for degradation with our self-produced laccases. The LC-MS and HPLC analytics showed that our laccases are able to degrade the hormonal substrates Estradiol, Esteron and Ethinyl estradiol [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We estabilished a method for the degradation of several compounds with the help of our self-produced laccases. The LC-MS and HPLC analytics showed that our laccases are able to degrade the hormonal substrates Estradiol, Esteron and Ethinyl estradiol [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] Successful immobilization of Bacillus pumilus DSM 27 -Laccase (CotA named BPUL) [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] Successful immobilization of ''Bacillus pumilus'' DSM 27 -Laccase (CotA named BPUL). [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] Successful immobilization of E. coli BL21- Laccase (CueO named ECOL) [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] Successful immobilization of ''E. coli'' BL21-Laccase (CueO named ECOL). [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] Successful immobilization of Trametes versicolor - Laccase (named TVEL0) [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] Successful immobilization of ''Trametes versicolor''-Laccase (named TVEL0). [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]

Revision as of 23:35, 26 September 2012


Project Achievments

Bielefeld2012 haken grau.jpg
We successfully produced purified and identified an active E. coli BL21-Laccase (CueO named ECOL). Results


Bielefeld2012 haken grau.jpg
We successfully designed a shuttle vector for site-directed integration in P. pastoris which allows the production and secretion of a protein of interest. Results


Bielefeld2012 haken grau.jpg
We successfully produced purified and identified an active Bacillus pumilus DSM 27-Laccase (CotA named BPUL). Results


Bielefeld2012 haken grau.jpg
We successfully produced, purified an active Thermus thermophilus HB27-Laccase (tthL named TTHL). Results


Bielefeld2012 haken grau.jpg
We successfully produced an active Bacillus halodurans C-125e-Laccase (Lbh1 named BHAL). Results


Bielefeld2012 haken grau.jpg
We estabilished a method for the degradation of several compounds with the help of our self-produced laccases. The LC-MS and HPLC analytics showed that our laccases are able to degrade the hormonal substrates Estradiol, Esteron and Ethinyl estradiol Results


Bielefeld2012 haken grau.jpg
Successful immobilization of Bacillus pumilus DSM 27 -Laccase (CotA named BPUL). Results


Bielefeld2012 haken grau.jpg
Successful immobilization of E. coli BL21-Laccase (CueO named ECOL). Results


Bielefeld2012 haken grau.jpg
Successful immobilization of Trametes versicolor-Laccase (named TVEL0). Results




55px Logo merck.jpg BioCircle.JPG Bielefeld2012 Evonik.jpg Bielefeld2012 Baxter.png Logo knauer.jpg Logo iit.jpg Bielefeld2012 BIEKUBA.jpg Logo biometra.jpg Logo bio-nrw.png Bielefeld2012 Logo ERASynbio.jpg