Team:Bielefeld-Germany/Labjournal/week3

From 2012.igem.org

(Difference between revisions)
(Tuesday May 15th)
(Week 3 (05/14 - 05/20/12))
 
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==Week 3 (05/14 - 05/20/12)==
==Week 3 (05/14 - 05/20/12)==
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=== weekly seminar ===
 
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* first lab service: Robert
 
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* our GFP, which we wanted to use for the summer school for pupils, does not work.
 
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* first competent cells have to be made: Julia S. and Robert.
 
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* Decision to buy a commercial laccase to establish the analytics and the enzyme activity tests.
 
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* our scientific exposé has to be translated into english: Malak
 
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* Last planning for our waver sell.
 
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* Julia S. is creating a vector for ''Pichia pastoris'' and is now searching for usable sequences.
 
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* The [http://www.bmbf.de/en/index.php BMBF] invites all german iGEM teams to Berlin to attend at the Biotechnologie2020+ strategy process.
 
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===Monday May 14th===
 
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===Tuesday May 15th===
 
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* '''Team Database''':
 
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**Creating the logically design for the database. For example for our table 'Eppi' we opt for the following logic:
 
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{|
 
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|eppi ( || ID INT(11),
 
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|-
 
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|      ||DataID INT(11),
 
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|-
 
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|      ||Caption TEXT,
 
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|-
 
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|      ||Content TEXT,
 
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|-
 
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| ||Experiment VARCHAR(255),
 
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|-
 
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| ||Where2 VARCHAR(255) , 
 
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|-
 
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| ||Location TEXT,
 
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|-
 
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| ||Coments TEXT,
 
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|-
 
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| ||Empty ENUM ('N', 'J'),
 
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|-
 
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| ||Create_User INT(11),
 
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|-
 
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| ||Creat_Date DATETIME,
 
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|-
 
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| ||Edit_Date DATETIME,
 
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|-
 
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| ||Delete ENUM ('N', 'J')
 
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|-
 
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| ||Delete_User INT(11),
 
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|-
 
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| ||Delete_Date DATETIME )
 
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|}
 
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===Wednesday May 16th===
 
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* '''Team Cloning of Bacterial Laccases''':
 
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:*For cloning our laccases we need pSB1C3 backbone. Therefore we we transformed [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] (pSB1C3 with RFP) in competent KRX cells.
 
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===Thursday May 17th===
 
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* '''Team Cloning of Bacterial Laccases''': Plasmid isolation of <partinfo>BBa_J04450</partinfo>.
 
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* '''Team Modeling''':
 
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**Meeting Mrs. Lutter, a mathematics prof. of our course of studies and looking for our first model of a metabolic pathway, finding out, that we don't need such a complex model. Start thinking that we want and what we need.
 
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===Friday May 18th===
 
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* '''Team Activity Tests''': Our [http://www.sigmaaldrich.com/catalog/product/sigma/53739?lang=de&region=DE ''T.versicolor'' laccase] and the [http://www.sigmaaldrich.com/catalog/product/sigma/53739?lang=de&region=DE ABTS] arrived! We couldn´t wait to start, so we set up the stock solutions we will need, such as sodium acetat buffer (pH 5), 10 mM ABTS and deluted laccase.
 
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===Saturday May 19th===
 
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===Sunday May 20th===
 
{{Team:Bielefeld/Sponsoren}}
{{Team:Bielefeld/Sponsoren}}

Latest revision as of 21:11, 25 September 2012


Week 3 (05/14 - 05/20/12)

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