Team:Bielefeld-Germany/Labjournal/week22

From 2012.igem.org

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(Monday September 24th)
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* '''Team Cultivation & Purification:'''[[File:Bielefeld2012_0924.jpg|300px|thumb|right|'''Figure 1: Cultivation from [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week21#Sunday_September_23rd 09/23]''' of ''E. coli'' KRX with <partinfo>BBa_K729006</partinfo> , ''E. coli'' KRX without plasmid (negative control) and with <partinfo>BBa_K863005</partinfo> (positive control). 60&nbsp;mL in 250&nbsp;mL flasks without baffles, [https://2012.igem.org/Team:Bielefeld-Germany/Protocols/Materials#Autoinduction_medium autoinduction medium], 60&nbsp;µg/mL chloramphenicol, 37&nbsp;°C for 12&nbsp;hours.]]
* '''Team Cultivation & Purification:'''[[File:Bielefeld2012_0924.jpg|300px|thumb|right|'''Figure 1: Cultivation from [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week21#Sunday_September_23rd 09/23]''' of ''E. coli'' KRX with <partinfo>BBa_K729006</partinfo> , ''E. coli'' KRX without plasmid (negative control) and with <partinfo>BBa_K863005</partinfo> (positive control). 60&nbsp;mL in 250&nbsp;mL flasks without baffles, [https://2012.igem.org/Team:Bielefeld-Germany/Protocols/Materials#Autoinduction_medium autoinduction medium], 60&nbsp;µg/mL chloramphenicol, 37&nbsp;°C for 12&nbsp;hours.]]
** For collaboration with [https://2012.igem.org/Team:University_College_London UCL London]: Made celldisruption via sonification and SDS-Page from lysates. The bands were analysed by MALDI-TOF.
** For collaboration with [https://2012.igem.org/Team:University_College_London UCL London]: Made celldisruption via sonification and SDS-Page from lysates. The bands were analysed by MALDI-TOF.
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Revision as of 18:20, 25 September 2012


Contents

Week 22 (09/24 - 09/30/12)

Monday September 24th

  • Team Cultivation & Purification:
    Figure 1: Cultivation from 09/23 of E. coli KRX with <partinfo>BBa_K729006</partinfo> , E. coli KRX without plasmid (negative control) and with <partinfo>BBa_K863005</partinfo> (positive control). 60 mL in 250 mL flasks without baffles, autoinduction medium, 60 µg/mL chloramphenicol, 37 °C for 12 hours.
    • For collaboration with UCL London: Made celldisruption via sonification and SDS-Page from lysates. The bands were analysed by MALDI-TOF.

















Tuesday September 25th

Wednesday September 26th

Thursday September 27th

Friday September 28st

Saturday September 29nd

Sunday September 30rd

Sunday


55px Logo merck.jpg BioCircle.JPG Bielefeld2012 Evonik.jpg Bielefeld2012 Baxter.png Logo knauer.jpg Logo iit.jpg Bielefeld2012 BIEKUBA.jpg Logo biometra.jpg Logo bio-nrw.png Bielefeld2012 Logo ERASynbio.jpg