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Team:Bielefeld-Germany/Labjournal/week16
From 2012.igem.org
Contents |
Week 16 (08/13 - 08/19/12)
Monday August 13th
Tuesday August 14th
- Team Arabidopsis Laccase: Today our cDNA took a bath in ethanol and got cleaned. We are pretty sure our next PCR will be a lot more successful. Check protocols for further information about the cDNA washing via ethanol precipitation.
Wednesday August 15th
- Team Wiki: We set up some more wiki rules. Today´s rules were about citations. We agreed von some standards that will for sure help make the wiki a little prettier. Have a look:
- Paper: Exampleman M et al. (2002). The example paper. The example journal (Volume): pages.
- Website: Name of website, URL, Index, date site visited
- Book: Examplewomen M et al. (1999). The example book. The example publisher (edition).
Next will be a standard for charts.
Thursday August 16th
Friday August 17th
Saturday August 18th
- Team bacterial laccases: Today we performed a ligation from the digestions we have done before. The ligation included of course the T7 promotor, the backbone pSB1C3 and the individual laccase genes. Ligation was performed with T4 ligase for 20 minutes at room temperature and stopped through a five minute incubation at 70°C.
Sunday August 19th
- Team Site Directed Mutagenesis:
Plasmidisolation of X.camp_2247- 1-4 and _3633 1-4 Test-restriktion of X.camp_2247 1-4 and _3633 1-4 with PstI for 3 hours 2247:1,2+4 no Mutation 3633:2+3 no Mutation. 2247-3 and 3633-1 only cut once (should be twice)
Picked 4 new colonys per site and plated the on CM-agar
Colony-PCR of 10 Colonies of T10 1,2,3,4 bands at 1100 - should be RFP 9 + 10 bands could be higher but not 1600 as needed… plated anyway for Test-restriktion.
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