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Team:Bielefeld-Germany/Labjournal/week12
From 2012.igem.org
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| - | * '''Team Modeling:''' We need a contact to a clarification plant to get information about clarification plant itself and perhaps to proof our cleaner with real probes. therefor we are calling Mr. | + | * '''Team Modeling:''' We need a contact to a clarification plant to get information about clarification plant itself and perhaps to proof our cleaner with real probes. therefor we are calling Mr. Bülter form the clarification plant Schloß Holte (near Bielefeld) and he invited us to present our projekt. |
===Saturday July 21st=== | ===Saturday July 21st=== | ||
===Sunday July 22nd=== | ===Sunday July 22nd=== | ||
Revision as of 20:17, 13 August 2012
Contents |
Labjournal
Week1 Week2 Week3 Week4 Week5 Week6 Week7 Week8 Week9 Week10 Week11 Week12 Week13 Week14 Week15
Week 12 (07/16 - 07/22/12)
Monday July 16th
- Team A. thaliana laccase: Our plants had a great time during the last weeks in the climate chamber. So today it was time for them to donate their seeds for RNA isolation, cDNA synthesis and a PCR (check protocols). We ran an additional sample with actin primers as a positive control. However both samples did not show any bands. Maybe the high salt concentration in our sample was responsible or the laccase concentration in the 1:10 deluted cDNA was too low. We will do some washing and try again.
Tuesday July 17th
- Team Cultivation/Purification: Second cultivation
Wednesday July 18th
Thursday July 19th
Friday July 20th
- Team Modeling: We need a contact to a clarification plant to get information about clarification plant itself and perhaps to proof our cleaner with real probes. therefor we are calling Mr. Bülter form the clarification plant Schloß Holte (near Bielefeld) and he invited us to present our projekt.
