Team:Bielefeld-Germany

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  • Our Plan
    TOXIC COMPOUNDS IN NATURAL WATER - A CASE FOR LACCASE
    The goal is to develop a biological filter using immobilized laccases to purify municipal and industrial wastewater from synthetic estrogens and other aromatic compounds.

    Click here to read more about our project

  • The Realization
    To realize our plans we first produce the laccases in the different clades and purify them. After this we immobilized and tested their acitivity.

    Click here to read more about our methodes...

  • The Potential
    While working with two different clarification plants we confirmed that our project has the opportunity to solve problems with micro contaminations.

    Click here to read more about the usage in a clarification plants and micro conterminations...

  • Laccases - Our Teammate
    Laccases have the ability to oxidize phenolic and nonphenolic lignin related compounds as well as highly recalcitrant environmental pollutants. This makes them very useful for applications concerning several biotechnological processes.

    Click here to read more about laccases...

Welcome

The birth control pill is a widespread contraception method. However, large amounts of these modified estrogens leave the body again in urine. The conventional methods in sewage treatment plants are unable to treat waste water sufficiently because the most frequently used estrogen ethinylestradiol is very difficult to break down. As a result, the hormone finds its way into rivers and lakes and accumulates in drinking water with serious consequences for fish and other aquatic life. These range from reproductive and severe developmental disorders to the formation of female sexual characteristics in males. The long-term consequences of increasing estrogen pollution for human beings are still largely unknown. Nonetheless, declining sperm counts and thereby increasing infertility in men living in industrial nations may well relate to this hormonal pollution. In addition, testicular and prostate cancers as well as osteoporosis could be a consequence of overly high concentrations of estrogen in the human body.

The goal of Bielefeld’s iGEM team is to develop a biological filter using immobilized laccases to purify municipal and industrial wastewater from synthetic estrogens and other aromatic compounds. Laccases are copper-containing oxidase enzymes found in many organisms, and one of their properties is the ability to break down a wide range of aromatic and phenolic compounds. For this purpose, genes of various bacterial and eucaryotic laccases should be isolated and expressed in ''Escherichia coli'' and ''Pichia pastoris''. The team is aiming to manufacture this enzyme economically and safely with the help of methods from synthetic biology. It should also be possible to extend the concept to other, in part poisonous and carcinogenic pollutants in drinking and waste water, as well as into industrial application, such as in paper and textile industries or even for bioremediation of contaminated soil.

A Case for Laccase!
iGEM Team Bielefeld 2012
Achievements

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Creation of a database to organize our probes.

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Production of an active E. coli Laccase.

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Production of an active B. pumilus Laccase.

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Production of an active T. thermophilus Laccase.

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Production of an active B. halodurans Laccase.

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Successful characterization of the activity of B. pumilus Laccase regarding the oxidization of ABTS under different conditions.

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Successful characterization of the activity of E. coli Laccase regarding the oxidization of ABTS under different conditions.

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Successful measurements of enzyme activity of T. thermophilus Laccase.

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Successful measurements of enzyme activity of B. halodurans Laccase.

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Successful immobilization of Trametes versicolor-Laccase (named TVEL0) and measurement of its activity.

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Successful immobilization of E. coli BL21-Laccase (CueO named ECOL) and measurement of its activity.

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Successful immobilization of Bacillus pumilus DSM 27-Laccase (CotA named BPUL) and measurement of its activity.

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Improved a BioBrick by clarifying its true function.

Interactions
Our Partners

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