Revision as of 14:55, 26 September 2012 by Juvoss (Talk | contribs)

  • The Add Script
    This script added a new entry to the database with a new DataID. In the script we insert a if/else statement which ensure that a location is given.
  • The Editing Script
    The editing script secured that every edtied entry is saved as a copy of the orinin entry. Just the newest copy of an entry is shown in the database. So it is possible to rebuild an entry if it is necessary.
  • The Deletion Script
    In the delete script we use the same secure system so that no entry is irretrievably deleted. Here aswell it is possible to rebuild an entry.
  • The Login Script
    In this script we use the entrys of the User table for the login. We check if the username and the password exists in this combination in the User table.
  • The Search Script
    With the search script you can isolate spcific entries of the table. When you use _ you can isolate word with one additional letter after the search text and with * it isolate word with any number of letters after the search text.

The birth control pill is a widespread contraception method. However, large amounts of these modified estrogens leave the body again in urine. The conventional methods in sewage treatment plants are unable to treat waste water sufficiently because the most frequently used estrogen ethinylestradiol is very difficult to break down. As a result, the hormone finds its way into rivers and lakes and accumulates in drinking water with serious consequences for fish and other aquatic life. These range from reproductive and severe developmental disorders to the formation of female sexual characteristics in males. The long-term consequences of increasing estrogen pollution for human beings are still largely unknown. Nonetheless, declining sperm counts and thereby increasing infertility in men living in industrial nations may well relate to this hormonal pollution. In addition, testicular and prostate cancers as well as osteoporosis could be a consequence of overly high concentrations of estrogen in the human body.

The goal of Bielefeld’s iGEM team is to develop a biological filter using immobilized laccases to purify municipal and industrial wastewater from synthetic estrogens and other aromatic compounds. Laccases are copper-containing oxidase enzymes found in many organisms, and one of their properties is the ability to break down a wide range of aromatic and phenolic compounds. For this purpose, genes of various bacterial and eucaryotic laccases should be isolated and expressed in ''Escherichia coli'' and ''Pichia pastoris''. The team is aiming to manufacture this enzyme economically and safely with the help of methods from synthetic biology. It should also be possible to extend the concept to other, in part poisonous and carcinogenic pollutants in drinking and waste water, as well as into industrial application, such as in paper and textile industries or even for bioremediation of contaminated soil.

iGEM Team Bielefeld 2012
1. Successful creation of a database to organize our probes. 2.
Our Partners

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