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  • Our Plan
    The goal is to develop a biological filter using immobilized laccases to purify municipal and industrial wastewater from synthetic estrogens and other aromatic compounds.

    Click here to

  • The Realization
    To realize our plans we first produce the laccases in the different clades and purify them. After this we immobilized and tested their acitivity.

    Click here to read more about our methodes...

  • The Potential
    While working with two different clarification plants we confirmed that our project has the opportunity to solve problems with micro contaminations.

    Link Zur Kläranlagenseite Click here to read more about the useag in a clarification plants and micro conterminations...

  • Laccases - Our Teammate
    Laccases have the ability to oxidize phenolic and nonphenolic lignin related compounds as well as highly recalcitrant environmental pollutants. This makes them very useful for applications concerning several biotechnological processes.

    Click here to read more about laccases...

  • The Search Script
    With the search script you can isolate spcific entries of the table. When you use _ you can isolate word with one additional letter after the search text and with * it isolate word with any number of letters after the search text.

The birth control pill is a widespread contraception method. However, large amounts of these modified estrogens leave the body again in urine. The conventional methods in sewage treatment plants are unable to treat waste water sufficiently because the most frequently used estrogen ethinylestradiol is very difficult to break down. As a result, the hormone finds its way into rivers and lakes and accumulates in drinking water with serious consequences for fish and other aquatic life. These range from reproductive and severe developmental disorders to the formation of female sexual characteristics in males. The long-term consequences of increasing estrogen pollution for human beings are still largely unknown. Nonetheless, declining sperm counts and thereby increasing infertility in men living in industrial nations may well relate to this hormonal pollution. In addition, testicular and prostate cancers as well as osteoporosis could be a consequence of overly high concentrations of estrogen in the human body.

The goal of Bielefeld’s iGEM team is to develop a biological filter using immobilized laccases to purify municipal and industrial wastewater from synthetic estrogens and other aromatic compounds. Laccases are copper-containing oxidase enzymes found in many organisms, and one of their properties is the ability to break down a wide range of aromatic and phenolic compounds. For this purpose, genes of various bacterial and eucaryotic laccases should be isolated and expressed in ''Escherichia coli'' and ''Pichia pastoris''. The team is aiming to manufacture this enzyme economically and safely with the help of methods from synthetic biology. It should also be possible to extend the concept to other, in part poisonous and carcinogenic pollutants in drinking and waste water, as well as into industrial application, such as in paper and textile industries or even for bioremediation of contaminated soil.

iGEM Team Bielefeld 2012
1. Successful creation of a database to organize our probes. 2.
Our Partners

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