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Team:Berkeley/Project/Localization/PromoterLibrary
From 2012.igem.org
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<td> pTDH3 </td> | <td> pTDH3 </td> | ||
<td width="205px"> <img src="https://static.igem.org/mediawiki/2012/6/65/YRC020_nrw_TDH3_ZRC1.jpg" width="200" > </td> | <td width="205px"> <img src="https://static.igem.org/mediawiki/2012/6/65/YRC020_nrw_TDH3_ZRC1.jpg" width="200" > </td> | ||
| - | <td width="205px"> This strain of yeast cells did not grow; | + | <td width="205px"> This strain of yeast cells did not grow; the over expression of the protein killed the cells. </td> |
<td width="205px"> <img src="https://static.igem.org/mediawiki/2012/b/bc/YRC030_nrw_TDH3_CP.jpg" width="200" > </td> | <td width="205px"> <img src="https://static.igem.org/mediawiki/2012/b/bc/YRC030_nrw_TDH3_CP.jpg" width="200" > </td> | ||
<td width="205px"> The RPL18B promoter already made the nucleus very bright, so we did not need a stronger promoter. </td> | <td width="205px"> The RPL18B promoter already made the nucleus very bright, so we did not need a stronger promoter. </td> | ||
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<td> pTEF1 </td> | <td> pTEF1 </td> | ||
<td><img src="https://static.igem.org/mediawiki/2012/6/66/YRC021_nrw_TEF1_ZRC1.jpg" width="200" > </td> | <td><img src="https://static.igem.org/mediawiki/2012/6/66/YRC021_nrw_TEF1_ZRC1.jpg" width="200" > </td> | ||
| - | <td> This strain of yeast cells did not grow; | + | <td> This strain of yeast cells did not grow; the over expression of the protein killed the cells. </td> |
<td><img src="https://static.igem.org/mediawiki/2012/4/42/YRC031_nrw_TEF1_CP.jpg" width="200" > </td> | <td><img src="https://static.igem.org/mediawiki/2012/4/42/YRC031_nrw_TEF1_CP.jpg" width="200" > </td> | ||
<td><img src="https://static.igem.org/mediawiki/2012/d/dc/YRC036_nrw_TEF1_Nucleus.jpg" width="200" > </td> | <td><img src="https://static.igem.org/mediawiki/2012/d/dc/YRC036_nrw_TEF1_Nucleus.jpg" width="200" > </td> | ||
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<td> pTDH3 </td> | <td> pTDH3 </td> | ||
<td width="205px"> <img src="https://static.igem.org/mediawiki/2012/3/37/YRC020_minmax_TDH3_ZRC1.jpg" width="200" > </td> | <td width="205px"> <img src="https://static.igem.org/mediawiki/2012/3/37/YRC020_minmax_TDH3_ZRC1.jpg" width="200" > </td> | ||
| - | <td width="205px"> This strain of yeast cells did not grow; | + | <td width="205px"> This strain of yeast cells did not grow; the over expression of the protein killed the cells. </td> |
<td width="205px"><img src="https://static.igem.org/mediawiki/2012/9/97/YRC030_minmax_TDH3_CP.jpg" width="200" > </td> | <td width="205px"><img src="https://static.igem.org/mediawiki/2012/9/97/YRC030_minmax_TDH3_CP.jpg" width="200" > </td> | ||
<td width="205px"> The RPL18B promoter already made the nucleus very bright, so we did not need a stronger promoter. </td> | <td width="205px"> The RPL18B promoter already made the nucleus very bright, so we did not need a stronger promoter. </td> | ||
| Line 124: | Line 121: | ||
<td> pTEF1 </td> | <td> pTEF1 </td> | ||
<td><img src="https://static.igem.org/mediawiki/2012/6/6b/YRC021_minmax_TEF1_ZRC1.jpg" width="200" ></td> | <td><img src="https://static.igem.org/mediawiki/2012/6/6b/YRC021_minmax_TEF1_ZRC1.jpg" width="200" ></td> | ||
| - | <td> This strain of yeast cells did not grow; | + | <td> This strain of yeast cells did not grow; the over expression of the protein killed the cells. </td> |
<td> <img src="https://static.igem.org/mediawiki/2012/e/e6/YRC031_minmax_TEF1_CP.jpg" width="200" > </td> | <td> <img src="https://static.igem.org/mediawiki/2012/e/e6/YRC031_minmax_TEF1_CP.jpg" width="200" > </td> | ||
<td> <img src="https://static.igem.org/mediawiki/2012/d/da/YRC036_minmax_TEF1_Nucleus.jpg" width="200" > </td> | <td> <img src="https://static.igem.org/mediawiki/2012/d/da/YRC036_minmax_TEF1_Nucleus.jpg" width="200" > </td> | ||
Revision as of 03:31, 4 October 2012
Narrow pixel brightness range (25-100):
| Vacuolar Membrane | Actin | Plasma Membrane | Nucleus | |
|---|---|---|---|---|
| pTDH3 |  |
This strain of yeast cells did not grow; the over expression of the protein killed the cells. |  |
The RPL18B promoter already made the nucleus very bright, so we did not need a stronger promoter. |
| pTEF1 |  |
This strain of yeast cells did not grow; the over expression of the protein killed the cells. |  |
 |
| pRPL18B |  |
 |
 |
 |
| pRNR2 |  |
 |
 |
 |
| pREV1 |  |
 |
 |
 |
Wide pixel brightness range (0-4095):
| Vacuolar Membrane | Actin | Plasma Membrane | Nucleus | |
|---|---|---|---|---|
| pTDH3 |  |
This strain of yeast cells did not grow; the over expression of the protein killed the cells. |  |
The RPL18B promoter already made the nucleus very bright, so we did not need a stronger promoter. |
| pTEF1 |  |
This strain of yeast cells did not grow; the over expression of the protein killed the cells. |  |
 |
| pRPL18B |  |
 |
 |
 |
| pRNR2 |  |
 |
 |
 |
| pREV1 |  |
 |
 |
 |
