Team:Austin Texas/Week of June 25 to July 2

From 2012.igem.org

Revision as of 17:15, 10 July 2012 by PeterOtoupal (Talk | contribs)

Contents

Notebook for week of June 25 to July 2

Design of converter plasmid

  • Razan
    • 6/26 - Tuesday: First assembly PCR for converter and control plasmid.
    • 6/29 - Friday: Overlap PCR on gel extracted first PCR reactions to get LasR+Terminator, GFP+Terminator, and Las Promoter+CRE.

Design of Reporter Plasmid

Spinach Aptamer

CBB5 Caffeine Inducible Promoters

  • Peter
    • 6/29 - Friday: Performed PCR using Ben's primers on ORF 1 site. Saved over weekend in 4C Fridge
    • 7/2 - Monday: Performed PCR using Ben's primers on BS_I20270 vector.

Inducible Odor