Team:Austin Texas/Week of June 25 to July 2

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Contents

Notebook for Week of June 25 to July 2

June 25

Design of converter plasmid

etc.

iGEM person

CBB5 Caffeine Inducible Promoters

Peter

  • Friday: Performed PCR using Ben's primers on ORF 1 site. Saved over weekend in 4C Fridge
  • Monday: Performed PCR using Ben's primers on BS_I20270 vector.
  • Tuesday: PCR purification on both PCRs. Gibson assembly of purified PCR. Gel Electrophoresis indicated that Gibson assembly was unsuccessful, as insert ORF 1 PCR was unsuccessful while vector PCR was successful. Erik independently performed PCR on the ORF 1. Used this, along with Dpn1 digested vector PCR to create a new Gibson.