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Team:Austin Texas/Week of June 25 to July 2
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= Notebook for week of June 25 to July 2 = | = Notebook for week of June 25 to July 2 = | ||
| - | + | == Design of converter plasmid == | |
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*Razan | *Razan | ||
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**6/29 - Friday: Overlap PCR on gel extracted first PCR reactions to get LasR+Terminator, GFP+Terminator, and Las Promoter+CRE. | **6/29 - Friday: Overlap PCR on gel extracted first PCR reactions to get LasR+Terminator, GFP+Terminator, and Las Promoter+CRE. | ||
| - | + | == Design of Reporter Plasmid == | |
| - | + | == Spinach Aptamer == | |
| - | + | == CBB5 Caffeine Inducible Promoters == | |
*Peter | *Peter | ||
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**7/3 - Tuesday: PCR purification on both PCRs. Gibson assembly of purified PCR. Gel Electrophoresis indicated that Gibson assembly was unsuccessful, as insert ORF 1 PCR was unsuccessful while vector PCR was successful. Erik independently performed PCR on the ORF 1. Used this, along with Dpn1 digested vector PCR to create a new Gibson. | **7/3 - Tuesday: PCR purification on both PCRs. Gibson assembly of purified PCR. Gel Electrophoresis indicated that Gibson assembly was unsuccessful, as insert ORF 1 PCR was unsuccessful while vector PCR was successful. Erik independently performed PCR on the ORF 1. Used this, along with Dpn1 digested vector PCR to create a new Gibson. | ||
| - | + | == Inducible Odor == | |
Revision as of 17:11, 10 July 2012
Contents |
Notebook for week of June 25 to July 2
Design of converter plasmid
- Razan
- 6/26 - Tuesday: First assembly PCR for converter and control plasmid.
- 6/29 - Friday: Overlap PCR on gel extracted first PCR reactions to get LasR+Terminator, GFP+Terminator, and Las Promoter+CRE.
Design of Reporter Plasmid
Spinach Aptamer
CBB5 Caffeine Inducible Promoters
- Peter
- 6/29 - Friday: Performed PCR using Ben's primers on ORF 1 site. Saved over weekend in 4C Fridge
- 7/2 - Monday: Performed PCR using Ben's primers on BS_I20270 vector.
- 7/3 - Tuesday: PCR purification on both PCRs. Gibson assembly of purified PCR. Gel Electrophoresis indicated that Gibson assembly was unsuccessful, as insert ORF 1 PCR was unsuccessful while vector PCR was successful. Erik independently performed PCR on the ORF 1. Used this, along with Dpn1 digested vector PCR to create a new Gibson.
