Team:Austin Texas/Week of June 25 to July 2
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PeterOtoupal (Talk | contribs) (→CBB5 Caffeine Inducible Promoters) |
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- | = Notebook for | + | = Notebook for week of June 25 to July 2 = |
== June 25 == | == June 25 == | ||
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iGEM person | iGEM person | ||
+ | |||
+ | === Design of Reporter Plasmid === | ||
+ | |||
+ | === Spinach Aptamer === | ||
=== CBB5 Caffeine Inducible Promoters === | === CBB5 Caffeine Inducible Promoters === | ||
- | + | *Peter | |
- | + | **6/29 - Friday: Performed PCR using Ben's primers on ORF 1 site. Saved over weekend in 4C Fridge | |
- | + | **7/2 - Monday: Performed PCR using Ben's primers on BS_I20270 vector. | |
- | + | **7/3 - Tuesday: PCR purification on both PCRs. Gibson assembly of purified PCR. Gel Electrophoresis indicated that Gibson assembly was unsuccessful, as insert ORF 1 PCR was unsuccessful while vector PCR was successful. Erik independently performed PCR on the ORF 1. Used this, along with Dpn1 digested vector PCR to create a new Gibson. | |
+ | |||
+ | === Inducible Odor === |
Revision as of 21:03, 3 July 2012
Contents |
Notebook for week of June 25 to July 2
June 25
Design of converter plasmid
etc.
iGEM person
Design of Reporter Plasmid
Spinach Aptamer
CBB5 Caffeine Inducible Promoters
- Peter
- 6/29 - Friday: Performed PCR using Ben's primers on ORF 1 site. Saved over weekend in 4C Fridge
- 7/2 - Monday: Performed PCR using Ben's primers on BS_I20270 vector.
- 7/3 - Tuesday: PCR purification on both PCRs. Gibson assembly of purified PCR. Gel Electrophoresis indicated that Gibson assembly was unsuccessful, as insert ORF 1 PCR was unsuccessful while vector PCR was successful. Erik independently performed PCR on the ORF 1. Used this, along with Dpn1 digested vector PCR to create a new Gibson.