Team:Austin Texas/Week of June 25 to July 2

From 2012.igem.org

(Difference between revisions)
(CBB5 Caffeine Inducible Promoters)
(Notebook for Week of June 25 to July 2)
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= Notebook for Week of June 25 to July 2 =
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= Notebook for week of June 25 to July 2 =
== June 25 ==
== June 25 ==
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iGEM person
iGEM person
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=== Design of Reporter Plasmid ===
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=== Spinach Aptamer ===
=== CBB5 Caffeine Inducible Promoters ===
=== CBB5 Caffeine Inducible Promoters ===
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;Peter
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*Peter
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:*Friday: Performed PCR using Ben's primers on ORF 1 site. Saved over weekend in 4C Fridge
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**6/29 - Friday: Performed PCR using Ben's primers on ORF 1 site. Saved over weekend in 4C Fridge
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:*Monday: Performed PCR using Ben's primers on BS_I20270 vector.
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**7/2  - Monday: Performed PCR using Ben's primers on BS_I20270 vector.
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:*Tuesday: PCR purification on both PCRs. Gibson assembly of purified PCR. Gel Electrophoresis indicated that Gibson assembly was unsuccessful, as insert ORF 1 PCR was unsuccessful while vector PCR was successful. Erik independently performed PCR on the ORF 1. Used this, along with Dpn1 digested vector PCR to create a new Gibson.
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**7/3  - Tuesday: PCR purification on both PCRs. Gibson assembly of purified PCR. Gel Electrophoresis indicated that Gibson assembly was unsuccessful, as insert ORF 1 PCR was unsuccessful while vector PCR was successful. Erik independently performed PCR on the ORF 1. Used this, along with Dpn1 digested vector PCR to create a new Gibson.
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=== Inducible Odor ===

Revision as of 21:03, 3 July 2012

Contents

Notebook for week of June 25 to July 2

June 25

Design of converter plasmid

etc.

iGEM person

Design of Reporter Plasmid

Spinach Aptamer

CBB5 Caffeine Inducible Promoters

  • Peter
    • 6/29 - Friday: Performed PCR using Ben's primers on ORF 1 site. Saved over weekend in 4C Fridge
    • 7/2 - Monday: Performed PCR using Ben's primers on BS_I20270 vector.
    • 7/3 - Tuesday: PCR purification on both PCRs. Gibson assembly of purified PCR. Gel Electrophoresis indicated that Gibson assembly was unsuccessful, as insert ORF 1 PCR was unsuccessful while vector PCR was successful. Erik independently performed PCR on the ORF 1. Used this, along with Dpn1 digested vector PCR to create a new Gibson.

Inducible Odor