Team:Austin Texas/Week of June 25 to July 2

From 2012.igem.org

(Difference between revisions)
(Created page with "= Notebook for Week of June 25 to July 2 = == June 25 == === Design of converter plasmid === etc. iGEM person")
(→Design of converter plasmid)
Line 8: Line 8:
iGEM person
iGEM person
 +
 +
=== CBB5 Caffeine Inducible Promoters ===
 +
 +
====Peter====
 +
:*Friday: Performed PCR using Ben's primers on ORF 1 site. Saved over weekend in 4C Fridge
 +
:*Monday: Performed PCR using Ben's primers on BS_I20270 vector.
 +
:*Tuesday: PCR purification on both PCRs. Gibson assembly of purified PCR. Gel Electrophoresis indicated that Gibson assembly was unsuccessful, as insert ORF 1 PCR was unsuccessful while vector PCR was successful. Erik independently performed PCR on the ORF 1. Used this, along with Dpn1 digested vector PCR to create a new Gibson.

Revision as of 20:58, 3 July 2012

Contents

Notebook for Week of June 25 to July 2

June 25

Design of converter plasmid

etc.

iGEM person

CBB5 Caffeine Inducible Promoters

Peter

  • Friday: Performed PCR using Ben's primers on ORF 1 site. Saved over weekend in 4C Fridge
  • Monday: Performed PCR using Ben's primers on BS_I20270 vector.
  • Tuesday: PCR purification on both PCRs. Gibson assembly of purified PCR. Gel Electrophoresis indicated that Gibson assembly was unsuccessful, as insert ORF 1 PCR was unsuccessful while vector PCR was successful. Erik independently performed PCR on the ORF 1. Used this, along with Dpn1 digested vector PCR to create a new Gibson.
Retrieved from "http://2012.igem.org/Team:Austin_Texas/Week_of_June_25_to_July_2"