Team:Austin Texas/Week of June 25 to July 2
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=== CBB5 Caffeine Inducible Promoters === | === CBB5 Caffeine Inducible Promoters === | ||
- | + | ;Peter | |
:*Friday: Performed PCR using Ben's primers on ORF 1 site. Saved over weekend in 4C Fridge | :*Friday: Performed PCR using Ben's primers on ORF 1 site. Saved over weekend in 4C Fridge | ||
:*Monday: Performed PCR using Ben's primers on BS_I20270 vector. | :*Monday: Performed PCR using Ben's primers on BS_I20270 vector. | ||
:*Tuesday: PCR purification on both PCRs. Gibson assembly of purified PCR. Gel Electrophoresis indicated that Gibson assembly was unsuccessful, as insert ORF 1 PCR was unsuccessful while vector PCR was successful. Erik independently performed PCR on the ORF 1. Used this, along with Dpn1 digested vector PCR to create a new Gibson. | :*Tuesday: PCR purification on both PCRs. Gibson assembly of purified PCR. Gel Electrophoresis indicated that Gibson assembly was unsuccessful, as insert ORF 1 PCR was unsuccessful while vector PCR was successful. Erik independently performed PCR on the ORF 1. Used this, along with Dpn1 digested vector PCR to create a new Gibson. |
Revision as of 20:59, 3 July 2012
Contents |
Notebook for Week of June 25 to July 2
June 25
Design of converter plasmid
etc.
iGEM person
CBB5 Caffeine Inducible Promoters
- Peter
- Friday: Performed PCR using Ben's primers on ORF 1 site. Saved over weekend in 4C Fridge
- Monday: Performed PCR using Ben's primers on BS_I20270 vector.
- Tuesday: PCR purification on both PCRs. Gibson assembly of purified PCR. Gel Electrophoresis indicated that Gibson assembly was unsuccessful, as insert ORF 1 PCR was unsuccessful while vector PCR was successful. Erik independently performed PCR on the ORF 1. Used this, along with Dpn1 digested vector PCR to create a new Gibson.