Team:Austin Texas/Week of July 9 to July 15
From 2012.igem.org
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**7/9- Gibson Assembly of Converter and Control plasmids | **7/9- Gibson Assembly of Converter and Control plasmids | ||
**7/10- Transformation of gibson failed and gel shows no correct bands | **7/10- Transformation of gibson failed and gel shows no correct bands | ||
- | **7/11- Redid Gibson assembly of Converter and Control plasmids with longer incubation time | + | **7/11- Redid Gibson assembly of Converter and Control plasmids with longer incubation time; same results. |
== Design of Reporter Plasmid == | == Design of Reporter Plasmid == |
Revision as of 17:32, 19 July 2012
Contents |
Notebook for week of July 9 to July 15
Design of Converter Plasmid
- Razan
- 7/9- Gibson Assembly of Converter and Control plasmids
- 7/10- Transformation of gibson failed and gel shows no correct bands
- 7/11- Redid Gibson assembly of Converter and Control plasmids with longer incubation time; same results.
Design of Reporter Plasmid
Spinach Aptamer
CBB5 Caffeine Inducible Promoters
- Peter
- 7/9 - Set up confirmation PCR of cultures. 1 for each culture, as well as 2 controls from CBB5 genomic DNA.
- 7/10 - Gel electrophoresis of confirmation PCR showed 2 successful Gibson product colonies, as well as both successful controls from CBB5 genomic DNA.