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Team:Austin Texas/Week of July 30 to August 5
From 2012.igem.org
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== Design of Converter Plasmid == | == Design of Converter Plasmid == | ||
| + | *Razan | ||
| + | **7/31- Sequenced DNA prepped from gibson transformation colonies- plasmids had errors in sequence. | ||
| + | **8/2- Redid gibson assembly reactions and re-transformed using filter before transformation. | ||
== Design of Reporter Plasmid == | == Design of Reporter Plasmid == | ||
| Line 30: | Line 33: | ||
*Peter | *Peter | ||
**7/31- Retry of cotransformation of PRA301-ndmApro LacZ reporter plasmid with orf1- 4,5,6 mini-preps. Used new miniprep from Ben, only 0.5 ul of each plasmid per transformation. 3 cotransformations, 4 single transformation for each plasmid. | **7/31- Retry of cotransformation of PRA301-ndmApro LacZ reporter plasmid with orf1- 4,5,6 mini-preps. Used new miniprep from Ben, only 0.5 ul of each plasmid per transformation. 3 cotransformations, 4 single transformation for each plasmid. | ||
| + | **8/1- 2 colonies picked from each cotransformation plate. | ||
| + | **8/2- Glycerol stock saved of 1st colony from 1st cotransformation plate (ORF1-4 + ndmApro) | ||
| + | **8/3- Transformation of ORF1-4 plasmid into TOP10 cells | ||
== Inducible Odor == | == Inducible Odor == | ||
Latest revision as of 01:08, 3 October 2012
Contents |
Notebook for week of July 30 to August 5
Design of Converter Plasmid
- Razan
- 7/31- Sequenced DNA prepped from gibson transformation colonies- plasmids had errors in sequence.
- 8/2- Redid gibson assembly reactions and re-transformed using filter before transformation.
Design of Reporter Plasmid
Spinach Aptamer
CBB5 Caffeine Inducible Promoters
- Peter
- 7/31- Retry of cotransformation of PRA301-ndmApro LacZ reporter plasmid with orf1- 4,5,6 mini-preps. Used new miniprep from Ben, only 0.5 ul of each plasmid per transformation. 3 cotransformations, 4 single transformation for each plasmid.
- 8/1- 2 colonies picked from each cotransformation plate.
- 8/2- Glycerol stock saved of 1st colony from 1st cotransformation plate (ORF1-4 + ndmApro)
- 8/3- Transformation of ORF1-4 plasmid into TOP10 cells
