Team:Austin Texas/Week of July 2 to July 9


Revision as of 17:16, 10 July 2012 by PeterOtoupal (Talk | contribs)


Notebook for week of July 2 to July 9

Design of Converter Plasmid

  • Razan
    • 7/2 - Overlap PCR of Las Promoter and GFP+Terminator for control plasmid.
    • 7/5 - Amplification PCR of gel extracted band from overlap PCR.

Design of Reporter Plasmid

  • Peter
    • 7/3 - Tuesday: PCR purification on both PCRs. Gibson assembly of purified PCR. Gel Electrophoresis indicated that Gibson assembly was unsuccessful, as insert ORF 1 PCR was unsuccessful while vector PCR was successful. Erik independently performed PCR on the ORF 1. Used this, along with Dpn1 digested vector PCR to create a new Gibson.

Spinach Aptamer

CBB5 Caffeine Inducible Promoters

Inducible Odor