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Team:Austin Texas/Week of July 2 to July 9
From 2012.igem.org
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| - | = Notebook for week of July 2 to July | + | = Notebook for week of July 2 to July 8 = |
== Design of Converter Plasmid == | == Design of Converter Plasmid == | ||
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*Peter | *Peter | ||
| - | **7/3 | + | **7/3 - PCR purification on both PCRs. Gibson assembly of purified PCR. Gel Electrophoresis indicated that Gibson assembly was unsuccessful, as insert ORF 1 PCR was unsuccessful while vector PCR was successful. Erik independently performed PCR on the ORF 1. Used this, along with Dpn1 digested vector PCR to create a new Gibson. |
| + | **7/5 - Made new lab SOC under Aruko's supervision. Transformation of supposedly successful Gibson assembly | ||
| + | **7/6 - Unsuccessful transformation. Made a new second attempt. | ||
| + | **7/7 - Collected 3 successful small colonies from Gibson product assembly, as well as three obviously larger colonies from Gibson control plate which grew. Made cultures for each picked colony. | ||
== Spinach Aptamer == | == Spinach Aptamer == | ||
Latest revision as of 17:21, 10 July 2012
Contents |
Notebook for week of July 2 to July 8
Design of Converter Plasmid
- Razan
- 7/2 - Overlap PCR of Las Promoter and GFP+Terminator for control plasmid.
- 7/5 - Amplification PCR of gel extracted band from overlap PCR.
Design of Reporter Plasmid
- Peter
- 7/3 - PCR purification on both PCRs. Gibson assembly of purified PCR. Gel Electrophoresis indicated that Gibson assembly was unsuccessful, as insert ORF 1 PCR was unsuccessful while vector PCR was successful. Erik independently performed PCR on the ORF 1. Used this, along with Dpn1 digested vector PCR to create a new Gibson.
- 7/5 - Made new lab SOC under Aruko's supervision. Transformation of supposedly successful Gibson assembly
- 7/6 - Unsuccessful transformation. Made a new second attempt.
- 7/7 - Collected 3 successful small colonies from Gibson product assembly, as well as three obviously larger colonies from Gibson control plate which grew. Made cultures for each picked colony.
