Team:Austin Texas/Week of July 2 to July 9
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- | = Notebook for week of July 2 to July | + | {{Template:Austin_Texas/Stylesheet}} |
+ | |||
+ | {| cellpadding="0" style="float: left;" "border: 0px solid darkgray;" | ||
+ | |- border="0" | ||
+ | |- align="center" | ||
+ | |<html> | ||
+ | <ul class="cssmenu"> | ||
+ | <li class="home"><a href="/Team:Austin_Texas" title="home"><span class="displace">Home</span></a></li> | ||
+ | <li class="team"><a href="/Team:Austin_Texas/Team" title="team"><span class="displace">Team</span></a></li> | ||
+ | <li class="official_team_profile"><a href="https://igem.org/Team.cgi?year=2012&team_name=Austin_Texas" title="official_team_profile"><span class="displace">Official Team Profile</span></a></li> | ||
+ | <li class="project"><a href="/Team:Austin_Texas/Project" title="project"><span class="displace">Project</span></a></li> | ||
+ | <li class="parts_submitted"><a href="/Team:Austin_Texas/Parts" title="parts_submitted"><span class="displace">Parts Submitted</span></a></li> | ||
+ | <li class="modelling"><a href="/Team:Austin_Texas/Modeling" title="modeling"><span class="displace">Modeling</span></a></li> | ||
+ | <li class="notebook"><a href="/Team:Austin_Texas/Notebook" class="selected" title="notebook"><span class="displace">Notebook</span></a></li> | ||
+ | <li class="safety"><a href="/Team:Austin_Texas/Safety" title="safety"></a></li> | ||
+ | <li class="attributions"><a href="/Team:Austin_Texas/Attributions" title="attributions"><span class="displace">Attributions</span></a></li> | ||
+ | </ul> | ||
+ | </html> | ||
+ | |} | ||
+ | |||
+ | = Notebook for week of July 2 to July 8 = | ||
== Design of Converter Plasmid == | == Design of Converter Plasmid == | ||
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*Peter | *Peter | ||
- | ** | + | **7/3 - PCR purification on both PCRs. Gibson assembly of purified PCR. Gel Electrophoresis indicated that Gibson assembly was unsuccessful, as insert ORF 1 PCR was unsuccessful while vector PCR was successful. Erik independently performed PCR on the ORF 1. Used this, along with Dpn1 digested vector PCR to create a new Gibson. |
+ | **7/5 - Made new lab SOC under Aruko's supervision. Transformation of supposedly successful Gibson assembly | ||
+ | **7/6 - Unsuccessful transformation. Made a new second attempt. | ||
+ | **7/7 - Collected 3 successful small colonies from Gibson product assembly, as well as three obviously larger colonies from Gibson control plate which grew. Made cultures for each picked colony. | ||
== Spinach Aptamer == | == Spinach Aptamer == |
Latest revision as of 17:21, 10 July 2012
Contents |
Notebook for week of July 2 to July 8
Design of Converter Plasmid
- Razan
- 7/2 - Overlap PCR of Las Promoter and GFP+Terminator for control plasmid.
- 7/5 - Amplification PCR of gel extracted band from overlap PCR.
Design of Reporter Plasmid
- Peter
- 7/3 - PCR purification on both PCRs. Gibson assembly of purified PCR. Gel Electrophoresis indicated that Gibson assembly was unsuccessful, as insert ORF 1 PCR was unsuccessful while vector PCR was successful. Erik independently performed PCR on the ORF 1. Used this, along with Dpn1 digested vector PCR to create a new Gibson.
- 7/5 - Made new lab SOC under Aruko's supervision. Transformation of supposedly successful Gibson assembly
- 7/6 - Unsuccessful transformation. Made a new second attempt.
- 7/7 - Collected 3 successful small colonies from Gibson product assembly, as well as three obviously larger colonies from Gibson control plate which grew. Made cultures for each picked colony.