Team:Austin Texas/Week of July 23 to July 29


Revision as of 21:26, 31 July 2012 by PeterOtoupal (Talk | contribs)


Notebook for week of July 23 to July 29

Design of Converter Plasmid

  • Razan
    • 7/24- Amplified pACYC backbone successfully with new primers for both control and converter plasmids.

Design of Reporter Plasmid

Spinach Aptamer

CBB5 Caffeine Inducible Promoters

  • Peter
    • 7/23- Cotransformation of PRA301-ndmApro LacZ reporter plasmid (with spectinomycin reporter) and ORF1 -4,5,6 plasmid minipreps. Ran out of PRA301-ndmApro plasmid, so only ORF1-4,5 were cotransformed. ORF1- 6 was transformed by itself as a control. Plated on kanamycin/XGal plates.
    • 7/24- Cotransformation failed. Likely due to too much plasmid, mixed with poor transformation.

Inducible Odor