Team:Austin Texas/Week of July 23 to July 29
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Notebook for week of July 23 to July 29
Design of Converter Plasmid
- Razan
- 7/24- Amplified pACYC backbone successfully with new primers for both control and converter plasmids.
Design of Reporter Plasmid
Spinach Aptamer
CBB5 Caffeine Inducible Promoters
- Peter
- 7/23- Cotransformation of PRA301-ndmApro LacZ reporter plasmid (with spectinomycin reporter) and ORF1 -4,5,6 plasmid minipreps. Ran out of PRA301-ndmApro plasmid, so only ORF1-4,5 were cotransformed. ORF1- 6 was transformed by itself as a control. Plated on kanamycin/XGal plates.
- 7/24- Cotransformation failed. Likely due to too much plasmid, mixed with poor transformation.